SIGNAL-TRANSDUCTION AND SPORULATION IN BACILLUS-SUBTILIS - AUTOPHOSPHORYLATION OF SPO0A, A SPORULATION INITIATION GENE-PRODUCT

被引：7

作者：

ASAYAMA, M ^{[1
]}

KOBAYASHI, Y ^{[1
]}

机构：

[1] TOKYO UNIV AGR & TECHNOL,FAC AGR,FUCHU,TOKYO 183,JAPAN

来源：

MOLECULAR & GENERAL GENETICS | 1993年 / 238卷 / 1-2期

关键词：

AUTOPHOSPHORYLATION; SIGNAL TRANSDUCTION; SPOOA; SPORULATION INITIATION; BACILLUS-SUBTILIS;

D O I：

10.1007/BF00279540

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spo0A is a positive/negative transcriptional regulator that plays a very important role in sporulation initiation in Bacillus subtilis. The N-terminal amino acid sequence of Spo0A is homologous to that of regulator proteins of the two-component regulatory systems involved in signal transduction in bacteria. Phosphorylation of Spo0A through a phosphorelay has been reported recently. In this study, we found that Spo0A is autophosphorylated in the presence of ATP and that an autophosphorylation-deficient Spo0A mutant is completely defective in initiating sporulation. These results suggest that Spo0A autophosphorylation is an essential event in the signal transduction process that controls sporulation in B. subtilis.

引用

页码：138 / 144

页数：7

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