BETA-AMYLASE ACTIVITY AS AN INDEX FOR GERMINATION POTENTIAL IN RICE

被引：39

作者：

NANDI, S ^{[1
]}

DAS, G ^{[1
]}

SENMANDI, S ^{[1
]}

机构：

[1] BOSE INST,SEED BIOL LAB,CALCUTTA 700009,W BENGAL,INDIA

来源：

ANNALS OF BOTANY | 1995年 / 75卷 / 05期

关键词：

RICE (ORYZA SATIVA L); GERMINATION; BETA-AMYLASE; ALPHA-AMYLASE; SEED VIGOR;

D O I：

10.1006/anbo.1995.1046

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Seeds of different vigour also differ in their germination ability. In rice (Oryza sativa), this difference was correlated with the level of incorporation of S-35-methionine into 25-60% ammonium sulphate precipitable material that was rich in amylase proteins. This protein fraction, from dry seeds, contained no cr-amylase activity. In contrast, beta-amylase activity was present in all seed stocks capable of 99% germination, although the level was lower in seeds that grew slowly when germinated. In low viability low vigour stock (i.e. extensively deteriorated seeds) beta-amylase activity was absent. Alpha-amylase activity in all stocks was detected only after 24 h from the start of imbibition. These results indicate that beta-amylase activity is a reliable indicator of the germination ability of rice seed stocks and of their vigour during germination.

引用

页码：463 / 467

页数：5

共 30 条

[1]

Ashford A.E., Gubler F., Mobilization of polysaccharide reserves from endosperm, Seed Physiology, 2, pp. 117-162, (1984)

[2]

Aspinall D., Paleg L.G., The deterioration of wheat embryo and endosperm function with age, Journal of Experimental Botany, 22, pp. 925-935, (1971)

[3]

Seed Vigour Testing Handbook, (1983)

[4]

Bernal-Lugo I., Beachy R.N., Varner J.E., The response of barley aleurone layer to gibberellic acid includes the transcription of new sequences, Biochemistry Biophysics Research Communications, 102, pp. 617-623, (1981)

[5]

Bewley J.D., Black M., Seeds: Physiology of Development and Germination, (1985)

[6]

Chrispeels M.J., Varner J.E., Hormonal changes of enzyme synthesis: On the mode of action of gibberellic acid and abscisin in alurone layers of barley, Plant Physiology, 42, pp. 1008-1016, (1967)

[7]

Das G., Sen-Mandi S., Utilisation of free fatty acids during germination of unaged and differentially aged wheat embryos, Indian Journal of Experimental Biology, 30, pp. 299-301, (1992)

[8]

Das G., Sen-Mandi S., Scutellar amylase activity in naturally aged and accelerated aged wheat seeds, Annals of Botany, 69, pp. 497-501, (1992)

[9]

Daussant J., Corrazier P., Biosynthesis and modification of α-and β-amylase in germinating wheat seeds, Federation of European Biochemical Societies Letters, 7, pp. 191-194, (1970)

[10]

Ganguli S., Sen-Mandi S., Effects of ageing on amylase activity and scuteller cell structure during imbibition in wheat seed, Annals of Botany, 71, pp. 411-416, (1993)

← 1 2 3 →