ADENOSINERGIC MODULATION OF CA1 NEURONAL TOLERANCE TO GLUCOSE DEPRIVATION IN ORGANOTYPIC HIPPOCAMPAL CULTURES

被引：36

作者：

HSU, SSF ^{[1
]}

NEWELL, DW ^{[1
]}

TUCKER, A ^{[1
]}

MALOUF, AT ^{[1
]}

WINN, HR ^{[1
]}

机构：

[1] UNIV WASHINGTON, HARBORVIEW MED CTR, SCH MED, DEPT NEUROL SURG, SEATTLE, WA 98104 USA

来源：

NEUROSCIENCE LETTERS | 1994年 / 178卷 / 02期

关键词：

ADENOSINE; ORGANOTYPIC HIPPOCAMPAL CULTURE; HYPOGLYCEMIA; GLUCOSE DEPRIVATION;

D O I：

10.1016/0304-3940(94)90756-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

zGlucose deprivation produced neuronal degeneration of CA1 pyramidal neurons in hippocampal slice cultures: The effects of the adenosine agonist cyclohexyladenosine (CHA) and antagonist cyclopentylxanthine (CPX) on CA1 neuronal loss following hypoglycemia was examined using propidium iodide fluorescence as an indicator of cell death. The intensity of propidium iodide fluorescence in hippocampal area CA1 was quantified using Optimas image analysis software. Following 2 or 3 h of glucose deprivation, CPX significantly enhanced injury in the CA1 region while CHA provided significant protection. These results suggest that adenosine plays an important role in endogenous neuronal protection during hypoglycemic injury, and also supports a role for the use of adenosine agonists as neuroprotective agents.

引用

页码：189 / 192

页数：4

共 22 条

[1] BIOLOGICAL DIFFERENCES BETWEEN ISCHEMIA, HYPOGLYCEMIA, AND EPILEPSY [J].

AUER, RN ;

SIESJO, BK .

ANNALS OF NEUROLOGY, 1988, 24 (06) :699-707

[2] ADENOSINE DECREASES ASPARTATE AND GLUTAMATE RELEASE FROM RAT HIPPOCAMPAL SLICES [J].

CORRADETTI, R ;

CONTE, GL ;

MORONI, F ;

PASSANI, MB ;

PEPEU, G .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1984, 104 (1-2) :19-26

[3]

DUNWIDDIE TV, 1989, J PHARMACOL EXP THER, V249, P31

[4]

FROTSCHER M, 1990, PROG BRAIN RES, V83, P323

[5] ORGANOTYPIC MONOLAYER-CULTURES OF NERVOUS-TISSUE [J].

GAHWILER, BH .

JOURNAL OF NEUROSCIENCE METHODS, 1981, 4 (04) :329-342

[6] DEVELOPMENT OF THE HIPPOCAMPUS INVITRO - CELL-TYPES, SYNAPSES AND RECEPTORS [J].

GAHWILER, BH .

NEUROSCIENCE, 1984, 11 (04) :751-760

[7] ADENOSINE REDUCES CORTICAL NEURONAL INJURY INDUCED BY OXYGEN OR GLUCOSE DEPRIVATION INVITRO [J].

GOLDBERG, MP ;

MONYER, H ;

WEISS, JH ;

CHOI, DW .

NEUROSCIENCE LETTERS, 1988, 89 (03) :323-327

[8] INFLUENCE OF HYPERGLYCEMIA ON CEREBRAL ADENOSINE PRODUCTION DURING ISCHEMIA AND REPERFUSION [J].

HSU, SSF ;

MENO, JR ;

ZHOU, JG ;

GORDON, EL ;

WINN, HR .

AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 261 (02) :H398-H403

[9] PHACLOFEN INHIBITION OF THE SLOW INHIBITORY POSTSYNAPTIC POTENTIAL IN HIPPOCAMPAL SLICE CULTURES - A POSSIBLE ROLE FOR THE GABA-B-MEDIATED INHIBITORY POSTSYNAPTIC POTENTIAL [J].

MALOUF, AT ;

ROBBINS, CA ;

SCHWARTZKROIN, PA .

NEUROSCIENCE, 1990, 35 (01) :53-61

[10] GLUCOSE DEPRIVATION NEURONAL INJURY IN CORTICAL CULTURE [J].

MONYER, H ;

GOLDBERG, MP ;

CHOI, DW .

BRAIN RESEARCH, 1989, 483 (02) :347-354

← 1 2 3 →