PHYTOCHELATIN PRODUCTION BY MARINE-PHYTOPLANKTON AT LOW FREE METAL-ION CONCENTRATIONS - LABORATORY STUDIES AND FIELD DATA FROM MASSACHUSETTS BAY

被引：106
作者：
AHNER, BA
PRICE, NM
MOREL, FMM
机构：
[1] R. M. Parsons Laboratory, Massachusetts Inst. of Technology, Cambridge
[2] Department of Biology, McGill University, Montreal
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 18期
关键词：
D O I：
10.1073/pnas.91.18.8433
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Phytochelatins are small metal-binding polypeptides synthesized by algae in response to high metal concentrations. Using a very sensitive HPLC method, we have quantified phytochelatins from phytoplankton in laboratory cultures at environmentally relevant metal concentrations and in marine field samples. Intracellular concentrations of phytochelatin, in the diatom Thalassiosira weissflogii, exhibit a distinct dose-response relation with free Cd2+ concentration in the medium-not with total Cd2+-and are detectable even when the free Cd2+ concentration is less than 1 pM. In Massachusetts Bay, phytochelatin levels (normalized to chlorophyll a) in the particulate fraction are similar to those measured in laboratory cultures exposed to picomolar free Cd2+ concentrations and exhibit a decreasing seaward trend. Incubations of natural samples with added Cd2+ confirmed the induction of the peptides by this metal. Ambient phytochelatin concentrations thus appear to provide a measure of the metal stress resulting from the complex mixture of trace metals and chelators in natural waters.
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页码：8433 / 8436
页数：4
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