EFFECT OF HUMAN DEFENSINS ON THE CYTOPLASMIC CA2+ CONTENT IN PLATELETS

被引：1
|
作者：
TKACHENKO, SB
KOKRYAKOV, VN
ASHMARIN, IP
GRACHEV, SV
KUBATIEV, AA
机构：
[1] RUSSIAN MED ACAD POSTGRAD EDUC,DEPT GEN PATHOL & PATHOL PHYSIOL,MOSCOW,RUSSIA
[2] MOSCOW MV LOMONOSOV STATE UNIV,DEPT BIOCHEM,MOSCOW,RUSSIA
[3] RUSSIAN ACAD MED SCI,INST EXPTL MED,DEPT PATHOPHYSIOL,ST PETERSBURG P-22,RUSSIA
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 1994年 / 118卷 / 12期
关键词：
DEFENSINS; PLATELETS; CA2+;
D O I：
10.1007/BF02445732
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The effect of the total fraction of human defensins (HNP-1, HNP-2, and HNP-3) on the cytoplasmic Ca2+ content ([Ca2+](i)) in the platelets of healthy donors was studied. At concentrations of 0.1-40 mu g/ml and an incubation time of 10 min defensins have no effect on [Ca2+](i) in platelets labeled with Fura-2AM. However, at higher concentrations (100 mu g/ml) they increased platelet [Ca2+](i). In addition, defensins (40 mu g/ml>) inhibited the Ca2+ increase in platelets induced by thrombin, adenosine diphosphate, and the lipopolysaccharide of S. typhimurium endotoxin. The most pronounced inhibitory effect was observed in a suspension of thrombin-stimulated platelets. It is shown that the effect of human defensins on the functional activity of platelets is due to the alterations in the intracellular Ca2+.
引用
收藏
页码：1291 / 1294
页数：4
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