DETERMINATION OF FENOVERINE IN TISSUE SAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：3

作者：

RATINEY, R ^{[1
]}

ASTIER, A ^{[1
]}

CHARIOT, P ^{[1
]}

机构：

[1] HOP HENRI MONDOR,DEPT TOXICOL,F-94000 CRETEIL,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 654卷 / 01期

关键词：

D O I：

10.1016/0378-4347(94)00016-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fenoverine is a spasmolytic, non-anticholinergic phenothiazine derivative that inhibits calcium channel currents. We describe a high-performance liquid chromatographic method for the determination of fenoverine in striated muscle, smooth, muscle, myocardium, and liver tissue. Reversed-phase liquid chromatography was performed on a 5 mum Nucleosil C18 column with acetonitrile-0.015 M phosphate buffer (28:72, v/v) as a mobile phase and detection with ultraviolet at 214 nm. The limit of quantitation of fenoverine in tissue samples was 25 ng injected. This method is well suited for the determination of fenoverine in various organs in animal experiments.

引用

页码：159 / 163

页数：5

共 13 条

[1]

BADER F, 1986, PHARMATHERAPEUTICA, V4, P422

[2]

BALDESSARINI R J, 1990, P383

[3]

BENAMOUZIG R, 1992, GASTROEN CLIN BIOL, V16, P719

[4] FENOVERINE - SMOOTH-MUSCLE SYNCHRONIZER FOR THE MANAGEMENT OF GASTROINTESTINAL CONDITIONS .2. A TRIMEBUTINE-CONTROLLED, DOUBLE-BLIND, CROSSOVER CLINICAL-EVALUATION [J].