THE SORPTION OF LYSOZYME AND RIBONUCLEASE ONTO FERROMAGNETIC NICKEL POWDER .1. ADSORPTION OF SINGLE COMPONENTS

被引：19

作者：

LIU, HS ^{[1
]}

WANG, YC ^{[1
]}

CHEN, WY ^{[1
]}

机构：

[1] NATL TAIWAN UNIV,DEPT CHEM ENGN,TAIPEI 10764,TAIWAN

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 1995年 / 5卷 / 1-2期

关键词：

ADSORPTION; FERROMAGNETIC NICKEL POWDER; LYSOZYME; RIBONUCLEASE;

D O I：

10.1016/0927-7765(95)98206-3

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The adsorption behavior of lysozyme and ribonuclease on nickel powder was studied to elucidate the effects of hydrophobicity, structural stability, and electrostatic forces. The results indicated that the equilibrium between proteins and nickel can be reached in about 20 min and some ''overshoot'' phenomena in adsorption were found. Maximal adsorption at the IEP, which could result in multilayer adsorption, was also noticed. A reduction in adsorption due to ionic shielding was observed for Cl- and SCN-, while the addition of ethanol promoted adsorption. FT-IR/ATR spectra were used to monitor the conformational changes during the adsorption process.

引用

页码：25 / 34

页数：10

共 55 条

[1] HEAVY-METALS REMOVAL BY HIGH-GRADIENT MAGNETIC SEPARATION [J].