FOLIAR UPTAKE OF CD BY PEA (PISUM-SATIVUM) AND SUGAR-BEET (BETA-VULGARIS)

Pea (Pisum sativum L. cv. Fenomen) and sugar beet (Beta vulgaris L. cv. Monohill) were cultivated in nutrient media without or with 10 muM CdCl2. Leaves of the same size and stage of developement, detached or still attached to the intact plants, were submerged into redistilled water containing 1 to 250 muM CdCl2. The uptake experiments were run for 1 to 8 h at pH 3.6 and 5.1. Cuticular transpiration rate, density of leaf and density of stomata were also measured. Percentage of open stomata was studied at different pH. Foliar uptake of Cd into the leaf is evident since Cd is transported from the exposed part of the pea leaves, through the petioles and into the stipules, and since the Cd concentration of the leaves increases with time and external Cd concentration. The foliar uptake depends on the permeability of the cuticular membrane, which is increased by a high intrinsic Cd level, which in turn enhances the foliar uptake of Cd in sugar beet. Higher cuticular permeability in pea than in sugar beet is shown by a 2.5 times higher cuticular transpiration rate and a 4 times lower density of leaf for pea, which causes a 7 times higher foliar uptake in pea than in sugar beet. Low pH decreases the net uptake of Cd, probably by an exchange reaction in the cutin and pectin of the cuticular membrane. Stomata are not directly involved in the Cd uptake, and the differences in the sum total of stomatal aperture area per unit leaf area is not related to differences in foliar uptake of Cd. Percentage of open stomata, calculated as average of both sides of the leaves, was not affected by changes in pH; but especially at high pH, proportionally more stomata were open on the adaxial than on the abaxial side.