THE TETRATRICOPEPTIDE REPEAT-DOMAIN OF THE PAS10 PROTEIN OF SACCHAROMYCES-CEREVISIAE IS ESSENTIAL FOR BINDING THE PEROXISOMAL TARGETING SIGNAL-SKL

The PAS10 gene was found in a two-hybrid screen for the isolation of genes encoding proteins which interact with the C-terminal peroxisomal targeting signal -SKL. The PAS10 protein is known to be involved in import of proteins into peroxisomes and to contain a tetratricopeptide repeat (TPR) domain. All TPR-containing proteins involved in diverse processes like mitosis or RNA-synthesis share the ability to interact with other proteins. Here we show that the PAS10 protein interacts in vivo with the C-terminal peroxisomal targeting signal. The part essential for this interaction contains the complete tetratricopeptide repeat domain. (C) 1994 Academic Press, Inc.