PLASMODIUM-FALCIPARUM - ISOLATION AND CHARACTERIZATION OF A 55-KDA PROTEASE WITH A CATHEPSIN D-LIKE ACTIVITY FROM PLASMODIUM-FALCIPARUM

被引：17

作者：

BAILLY, E

SAVEL, J

MAHOUY, G

JAUREGUIBERRY, G

机构：

[1] HOP CLAUDE BERNARD,INSERM,U13,10 AVE PORTE AUBERVILLIERS,F-75019 PARIS,FRANCE

[2] HOP ST LOUIS,CTR HAYEM,IMMUNOMODULAT LAB,F-75010 PARIS,FRANCE

来源：

EXPERIMENTAL PARASITOLOGY | 1991年 / 72卷 / 03期

关键词：

PLASMODIUM-FALCIPARUM; CATHEPSIN-D; ELECTROPHORESIS; WESTERN BLOTTING; CHLOROQUINE SENSITIVITY; 4-(2 HYDROXYETHYL)-1-PIPERAZINE ETHANE SULFONIC ACID (HEPES); PHOSPHATE-BUFFER SALINE (PBS); FALCIPARUM CULTURE MBA NUMBER-29 (FCM29); TRIS(HYDROXYETHYL-AMINOETHANE) (TRIS); ETHYLENE-DIAMINE TETRACETIC ACID (EDTA); RED BLOOD CELL (RBC); PEPSTATIN-A (PA); PHENYLMETHYLSULFONYL FLUORIDE (PMSF); N-ETHYLMALEIMIDE (NEM); N-ALPHA-TOSYL-L-LYSINE-CHLOROMETHYL-KETONE; CATHEPSIN-D ANTISERUM (CDA);

D O I：

10.1016/0014-4894(91)90147-O

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Native electrophoresis followed by imprint digest method using hemoglobin as substrate allowed the detection of parasite hemoglobinase activity at acidic pH (3.9 to 5). This protease was inhibited specifically by pepstatin A and insensitive to other protease inhibitors. The molecular weight determination using modified SDS-PAGE followed by imprint digest method, demonstrated a single area of activity at 55-58 kDa, similar to cathepsin D characterized in eucaryotic cells. The parasitic origin has been shown by radiolabeling experiments with [35S]-methionine. The 55-kDa protein was immunoprecipitated by a rabbit anti-cathepsin D serum. © 1991.

引用

页码：278 / 284

页数：7

共 24 条

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