QUANTITATION OF A MITOCHONDRIAL-DNA DELETION IN PARKINSONS-DISEASE

被引：85

作者：

MANN, VM

COOPER, JM

SCHAPIRA, AHV

机构：

[1] ROYAL FREE HOSP,SCH MED,DEPT PROT & MOLEC BIOL,LONDON NW3 2PF,ENGLAND

[2] INST NEUROL,LONDON WC1N 3BG,ENGLAND

来源：

FEBS LETTERS | 1992年 / 299卷 / 03期

关键词：

NADH COQ1 REDUCTASE; PARKINSONS DISEASE; MITOCHONDRION; MITOCHONDRIAL DNA;

D O I：

10.1016/0014-5793(92)80118-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 5 kilobase deletion in mitochondrial DNA (mtDNA) has been reported to be responsible for the specific complex I deficiency in the substantia nigra (SN) of the Parkinson's disease (PD) brain. We have studied mitochondrial respiratory chain function in the SN from control and PD subjects, and analysed mtDNA, extracted from the same tissues, by Southern blot and the polymerase chain reaction (PCR). Quantitation of the levels of the deletion indicate that it does not contribute to the pathogenesis of PD nor to a complex I deficiency but seems likely to be an age-related observation.

引用

收藏

页码：218 / 222

页数：5

相关论文

共 23 条

[1] REPLICATION OF ANIMAL MITOCHONDRIAL-DNA [J].

CLAYTON, DA .

CELL, 1982, 28 (04) :693-705

[2] DETECTION OF A SPECIFIC MITOCHONDRIAL-DNA DELETION IN TISSUES OF OLDER HUMANS [J].

CORTOPASSI, GA ;

ARNHEIM, N .

NUCLEIC ACIDS RESEARCH, 1990, 18 (23) :6927-6933

[3] A DOPAMINERGIC CELL-LINE VARIANT RESISTANT TO THE NEUROTOXIN 1-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE [J].

DENTON, T ;

HOWARD, BD .

JOURNAL OF NEUROCHEMISTRY, 1987, 49 (02) :622-630

[4] ANALYSIS OF CYTOKINE MESSENGER-RNA AND DNA - DETECTION AND QUANTITATION BY COMPETITIVE POLYMERASE CHAIN-REACTION [J].

GILLILAND, G ;

PERRIN, S ;

BLANCHARD, K ;

BUNN, HF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (07) :2725-2729

[5] A MUTATION IN THE TRANSFER RNALEU(UUR) GENE ASSOCIATED WITH THE MELAS SUBGROUP OF MITOCHONDRIAL ENCEPHALOMYOPATHIES [J].

GOTO, Y ;

NONAKA, I ;

HORAI, S .

NATURE, 1990, 348 (6302) :651-653

[6] DELETIONS OF MUSCLE MITOCHONDRIAL-DNA IN MITOCHONDRIAL MYOPATHIES - SEQUENCE-ANALYSIS AND POSSIBLE MECHANISMS [J].

HOLT, IJ ;

HARDING, AE ;

MORGANHUGHES, JA .

NUCLEIC ACIDS RESEARCH, 1989, 17 (12) :4465-4469

[7] MITOCHONDRIAL MYOPATHIES - CLINICAL AND BIOCHEMICAL FEATURES OF 30 PATIENTS WITH MAJOR DELETIONS OF MUSCLE MITOCHONDRIAL-DNA [J].

HOLT, IJ ;

HARDING, AE ;

COOPER, JM ;

SCHAPIRA, AHV ;

TOSCANO, A ;

CLARK, JB ;

MORGANHUGHES, JA .

ANNALS OF NEUROLOGY, 1989, 26 (06) :699-708

[8] INCREASE OF DELETED MITOCHONDRIAL-DNA IN THE STRIATUM IN PARKINSONS-DISEASE AND SENESCENCE [J].

IKEBE, S ;

TANAKA, M ;

OHNO, K ;

SATO, W ;

HATTORI, K ;

KONDO, T ;

MIZUNO, Y ;

OZAWA, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 170 (03) :1044-1048

[9] PARKINSONISM-INDUCING NEUROTOXIN, N-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE - UPTAKE OF THE METABOLITE N-METHYL-4-PHENYLPYRIDINE BY DOPAMINE NEURONS EXPLAINS SELECTIVE TOXICITY [J].

JAVITCH, JA ;

DAMATO, RJ ;

STRITTMATTER, SM ;

SNYDER, SH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (07) :2173-2177

[10] CHRONIC PARKINSONISM IN HUMANS DUE TO A PRODUCT OF MEPERIDINE-ANALOG SYNTHESIS [J].

LANGSTON, JW ;

BALLARD, P ;

TETRUD, JW ;

IRWIN, I .

SCIENCE, 1983, 219 (4587) :979-980