DISTRIBUTION OF NITRIC-OXIDE SYNTHASE CONTAINING ELEMENTS IN THE FELINE SUBMANDIBULAR-GLAND

被引:45
作者
LOHINAI, Z
SZEKELY, AD
SOOS, L
FEHER, E
机构
[1] SEMMELWEIS UNIV MED,DEPT ANAT 1,H-1450 BUDAPEST,HUNGARY
[2] SEMMELWEIS UNIV MED,DEPT EXPTL RES,H-1085 BUDAPEST,HUNGARY
[3] SEMMELWEIS UNIV MED,INST PHYSIOL 2,H-1085 BUDAPEST,HUNGARY
关键词
NADPH-DIAPHORASE; NITRIC OXIDE SYNTHASE; HISTOCHEMISTRY; IMMUNOCYTOCHEMISTRY; SUBMANDIBULAR GLAND; CAT;
D O I
10.1016/0304-3940(95)11594-M
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Combined nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry and nitric oxide synthase (NOS) immunocytochemistry were used to study the distribution of nitric oxide synthesizing elements in the cat submandibular gland. A large number of thin varicose fibres, with intense staining for both markers, were seen around or in close contact with the acini. Some of the stained nerve fibres were associated with intra- and interlobular salivary ducts and blood vessels. All neurones in the submandibular ganglia showed intense staining for both NADPH-d and NOS. The epithelial layer of the salivary ductal branches and the endothelial lining of blood vessels were NOS immunonegative but NADPH-d positive. Our results suggest that NO might act as a neurotransmitter in the regulation of blood flow and secretion in the submandibular salivary gland.
引用
收藏
页码:9 / 12
页数:4
相关论文
共 19 条
[1]  
Alm, Larsson, Ekblad, Sundler, Andersson, Immunohistochemical localization of peripheral nitric oxide synthase-containing nerves using antibodies raised against synthesized C- and N-terminal fragments of a cloned enzyme from rat brain, Acta Physiol. Scand., 148, pp. 421-429, (1993)
[2]  
Berezin, Snyder, Bredt, Daniel, Ultrastructural localization of nitric oxide synthase in canine small intestine and colon, Am. J. Physiol., 266, pp. C981-C989, (1994)
[3]  
Bodis, Haregewoin, Evidence for the release and possible neural regulation of nitric oxide in human saliva, Biochem. Biophys. Res. Commun., 194, pp. 347-350, (1993)
[4]  
De-Giorgio, Parodi, Brecha, Brunicardi, Becker, Go, Sternini, Nitric oxide producing neurons in the monkey and human digestive system, J. Comp. Neurol., 342, pp. 619-627, (1994)
[5]  
Edwards, Garrett, Endothelium-derived vasodilator responses to sympathetic stimulation of the submandibular gland in the cat, J. Physiol., 456, pp. 491-501, (1992)
[6]  
Edwards, Garrett, Nitric oxide-related vasodilator responses to parasympathetic stimulation of the submandibular gland in the cat, J. Physiol., 464, pp. 379-392, (1993)
[7]  
Faussone-Pellegrini, Bacci, Pantalone, Cortesini, Mayer, Nitric oxide synthase immunoreactivity in the human ileocecal region, Neurosci. Lett., 170, pp. 261-265, (1994)
[8]  
Gabbott, Bacon, Histochemical localization of NADPH-dependent diaphorase (nitric oxide synthase) activity in vascular endothelial cells in the rat brain, Neuroscience, 57, pp. 79-95, (1993)
[9]  
Grozdanovic, Baumgarten, Bruning, Histochemistry of NADPH-diaphorase, a marker for neuronal nitric oxide synthase, in the peripheral autonomic nervous system of the mouse, Neuroscience, 48, pp. 225-239, (1992)
[10]  
Hauser-Kronberger, Saria, Hacker, Peptidergic innervation of human salivary glands (parotid gland and submandibular gland), Hals-, Nasen- und Ohrheilkunde, 40, pp. 429-436, (1992)