GENE-EXPRESSION OF A THERMOSTABLE BETA-GALACTOSIDASE IN MAMMALIAN-CELLS AND ITS APPLICATION IN ASSAYS OF EUKARYOTIC PROMOTER ACTIVITY

被引：0
作者：
CANNIO, R
DEPASCALE, D
ROSSI, M
BARTOLUCCI, S
机构：
[1] UNIV NAPLES 2,DIPARTIMENTO CHIM ORGAN & BIOL,I-80134 NAPLES,ITALY
[2] CNR,IST BIOCHIM PROT & ENZIMOL,I-80125 NAPLES,ITALY
来源：
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 1994年 / 19卷
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The gene (lacS) encoding a thermostable beta-galactosidase enzyme (Ss beta-gal) from the archaebacterium Sulfolobus solfataricus has been cloned and expressed in simian CV1 and murine NIH3T3 cell lines. The recombinant protein is an active enzyme that shows the same properties of thermophilicity and thermostability as the wild-type Ss beta-gal and has no cytotoxic effect on the host cells. Its possible use as a reporter gene is also proposed, and a comparison with other reporter gene systems is made.
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页码：233 / 242
页数：10
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