HIGHLY BIOLUMINESCENT BACILLUS-SUBTILIS OBTAINED THROUGH HIGH-LEVEL EXPRESSION OF A LUXAB FUSION GENE

被引：20
作者：
JACOBS, M
HILL, PJ
STEWART, GSAB
机构：
[1] UNIV NOTTINGHAM,FAC AGR & FOOD SCI,DEPT APPL BIOCHEM & FOOD SCI,LOUGHBOROUGH LE12 5RD,LEICS,ENGLAND
[2] TECH UNIV DENMARK,DEPT MICROBIOL,DK-2800 LYNGBY,DENMARK
来源：
MOLECULAR & GENERAL GENETICS | 1991年 / 230卷 / 1-2期
关键词：
BACILLUS-SUBTILIS; LUCIFERASE; BIOLUMINESCENCE; TRANSLATIONAL COUPLING;
D O I：
10.1007/BF00290675
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bioluminescence levels comparable to those achievable in Escherichia coli have yet to be obtained from luxAB expression in gram-positive bacteria. In this communication we describe the gene engineering required to generate a highly bioluminescent derivative of Bacillus subtilis. The combination of a powerful promoter, P(xyn), a fusion derivative of luxAB from Vibrio harveyi and translational coupling have overcome the previously reported limitations in luxAB expression. The implications for highly bioluminescent gram-positive organisms are discussed.
引用
收藏
页码：251 / 256
页数：6
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