HIGHLY BIOLUMINESCENT BACILLUS-SUBTILIS OBTAINED THROUGH HIGH-LEVEL EXPRESSION OF A LUXAB FUSION GENE

被引：20

作者：

JACOBS, M

HILL, PJ

STEWART, GSAB

机构：

[1] UNIV NOTTINGHAM,FAC AGR & FOOD SCI,DEPT APPL BIOCHEM & FOOD SCI,LOUGHBOROUGH LE12 5RD,LEICS,ENGLAND

[2] TECH UNIV DENMARK,DEPT MICROBIOL,DK-2800 LYNGBY,DENMARK

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 230卷 / 1-2期

关键词：

BACILLUS-SUBTILIS; LUCIFERASE; BIOLUMINESCENCE; TRANSLATIONAL COUPLING;

D O I：

10.1007/BF00290675

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bioluminescence levels comparable to those achievable in Escherichia coli have yet to be obtained from luxAB expression in gram-positive bacteria. In this communication we describe the gene engineering required to generate a highly bioluminescent derivative of Bacillus subtilis. The combination of a powerful promoter, P(xyn), a fusion derivative of luxAB from Vibrio harveyi and translational coupling have overcome the previously reported limitations in luxAB expression. The implications for highly bioluminescent gram-positive organisms are discussed.

引用

页码：251 / 256

页数：6

共 45 条

[31] Cloned Bacillus subtilis alkaline protease (aprA) gene showing high level of keratinolytic activity
Zaghloul, TI
[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1998, 70-2 (1) : 199 - 205
[32] High-level production of a cold-active B-mannanase from Bacillus subtilis Bs5 and its molecular cloning and expression
Huang, Jun Li
Bao, Ling Xiao
Zou, Han Yan
Che, Shu Gang
Wang, Gui Xue
[J]. MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY, 2012, 27 (04) : 147 - 153
[33] ANALYSIS OF AN INSERTIONAL OPERATOR MUTATION (GNTOI) THAT AFFECTS THE EXPRESSION LEVEL OF THE BACILLUS-SUBTILIS GNT OPERON, AND CHARACTERIZATION OF GNTOI SUPPRESSOR MUTATIONS
YOSHIDA, K
MIWA, Y
OHMORI, H
FUJITA, Y
[J]. MOLECULAR & GENERAL GENETICS, 1995, 248 (05): : 583 - 591
[34] SPORULATION OPERON SPOIVF AND THE CHARACTERIZATION OF MUTATIONS THAT UNCOUPLE MOTHER-CELL FROM FORESPORE GENE-EXPRESSION IN BACILLUS-SUBTILIS
CUTTING, S
ROELS, S
LOSICK, R
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1991, 221 (04) : 1237 - 1256
[35] High-level production, purification and characterization of a thermostable β-mannanase from the newly isolated Bacillus subtilis WY34
Jiang, Zhengqiang
Wei, Yun
Li, Daoyi
Li, Lite
Chai, Pingping
Kusakabe, Isao
[J]. CARBOHYDRATE POLYMERS, 2006, 66 (01) : 88 - 96
[36] High-level secretion of a recombinant protein to the culture medium with a Bacillus subtilis twin-arginine translocation system in Escherichia coli
Albiniak, Anna M.
Matos, Cristina F. R. O.
Branston, Steven D.
Freedman, Robert B.
Keshavarz-Moore, Eli
Robinson, Colin
[J]. FEBS JOURNAL, 2013, 280 (16) : 3810 - 3821
[37] MODIFICATION OF CENTRAL METABOLIC PATHWAY IN ESCHERICHIA-COLI TO REDUCE ACETATE ACCUMULATION BY HETEROLOGOUS EXPRESSION OF THE BACILLUS-SUBTILIS ACETOLACTATE SYNTHASE GENE
ARISTIDOU, AA
SAN, KY
BENNETT, GN
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1994, 44 (08) : 944 - 951
[38] High-Level 5-Methyltetrahydrofolate Bioproduction in Bacillus subtilis by Combining Modular Engineering and Transcriptomics-Guided Global Metabolic Regulation
Yang, Han
Yang, Jinning
Liu, Cheng
Lv, Xueqin
Liu, Long
Li, Jianghua
Du, Guocheng
Chen, Jian
Liu, Yanfeng
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2022, 70 (19) : 5849 - 5859
[39] Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch
Guo, Haoyu
Tian, Rongzhen
Wu, Yaokang
Lv, Xueqin
Li, Jianghua
Liu, Long
Du, Guocheng
Chen, Jian
Liu, Yanfeng
[J]. SYNTHETIC AND SYSTEMS BIOTECHNOLOGY, 2024, 9 (03) : 577 - 585
[40] High-level intra- and extra-cellular production of D-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis
Chen, Jingqi
Zhu, Yueming
Fu, Gang
Song, Yafeng
Jin, Zhaoxia
Sun, Yuanxia
Zhang, Dawei
[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2016, 43 (11) : 1577 - 1591

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