HIGHLY BIOLUMINESCENT BACILLUS-SUBTILIS OBTAINED THROUGH HIGH-LEVEL EXPRESSION OF A LUXAB FUSION GENE

被引：20

作者：

JACOBS, M

HILL, PJ

STEWART, GSAB

机构：

[1] UNIV NOTTINGHAM,FAC AGR & FOOD SCI,DEPT APPL BIOCHEM & FOOD SCI,LOUGHBOROUGH LE12 5RD,LEICS,ENGLAND

[2] TECH UNIV DENMARK,DEPT MICROBIOL,DK-2800 LYNGBY,DENMARK

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 230卷 / 1-2期

关键词：

BACILLUS-SUBTILIS; LUCIFERASE; BIOLUMINESCENCE; TRANSLATIONAL COUPLING;

D O I：

10.1007/BF00290675

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bioluminescence levels comparable to those achievable in Escherichia coli have yet to be obtained from luxAB expression in gram-positive bacteria. In this communication we describe the gene engineering required to generate a highly bioluminescent derivative of Bacillus subtilis. The combination of a powerful promoter, P(xyn), a fusion derivative of luxAB from Vibrio harveyi and translational coupling have overcome the previously reported limitations in luxAB expression. The implications for highly bioluminescent gram-positive organisms are discussed.

引用

页码：251 / 256

页数：6

共 45 条

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