EFFECTS OF HYPOTHALAMIC PEPTIDES ON ELECTRICAL-ACTIVITY AND MEMBRANE CURRENTS OF PATCH PERFORATED CLAMPED GH3 ANTERIOR-PITUITARY-CELLS

被引：30
作者：
BARROS, F
DELGADO, LM
MACIA, C
DELAPENA, P
机构：
[1] Departamento de Biologia Funcional, Area de Bioquimica, Facultad de Medicina
来源：
FEBS LETTERS | 1991年 / 279卷 / 01期
关键词：
PERFORATED-PATCH RECORDING; THYROTROPIN RELEASING HORMONE; SOMATOSTATIN; ANTERIOR PITUITARY;
D O I：
10.1016/0014-5793(91)80243-V
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
'Perforated-patch' recordings of rat anterior pituitary GH3 cells allow long and stable monitoring of electrical activity and membrane currents. Under current clamp conditions, the biphasic effect of thryotropin releasing hormone (TRH) consisting of a transient hyperpolarization followed by a longer phase of increased action potential frequency is fully preserved. Somatostatin suppresses action potential activity and antagonizes the second phase of enhanced spiking caused by TRH. Voltage clamp records of isolated currents indicate that TRH affects calcium-dependent potassium currents, but does not alter either voltage-dependent potassium or calcium currents at times and concentrations at which the electrical activity is increased.
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收藏
页码：33 / 37
页数：5
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