CDNA CLONING, EXPRESSION AND PRIMARY STRUCTURE OF PAR-J-I, A MAJOR ALLERGEN OF PARIETARIA-JUDAICA POLLEN

被引：62

作者：

COSTA, MA ^{[1
]}

COLOMBO, P ^{[1
]}

IZZO, V ^{[1
]}

KENNEDY, H ^{[1
]}

VENTURELLA, S ^{[1
]}

COCCHIARA, R ^{[1
]}

MISTRELLO, G ^{[1
]}

FALAGIANI, P ^{[1
]}

GERACI, D ^{[1
]}

机构：

[1] LAB FARMACEUT LOFARMA,MILAN,ITALY

来源：

FEBS LETTERS | 1994年 / 341卷 / 2-3期

关键词：

CDNA; IGE ANTIBODY; PRIMARY STRUCTURE; POLLINOSIS; RECOMBINANT ALLERGEN; PARIETARIA-JUDAICA;

D O I：

10.1016/0014-5793(94)80453-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 659 bp cDNA clone coding for an allergen of Pj pollen has been isolated from a lambda gt11 library, and its DNA sequence determined. The cDNA insert showed an open reading frame of 429 bp coding for an allergenic protein of 14,866 Da and a deduced amino acid sequence containing 143 residues. The expressed recombinant protein represented the major allergen Par j I since it reacted with 95% of the sera from Pj-allergic patients (n = 22) and with two Par j I-specific monoclonal antibodies. No similarity with other known DNA and protein sequences has been detected.

引用

页码：182 / 186

页数：5

共 28 条

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