CRYSTAL-STRUCTURE OF RECOMBINANT HUMAN T-CELL CYCLOPHILIN-A AT 2.5-A RESOLUTION

被引：192

作者：

KE, HM ^{[1
]}

ZYDOWSKY, LD ^{[1
]}

LIU, J ^{[1
]}

WALSH, CT ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOLEC PHARMACOL,BOSTON,MA 02115

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 21期

关键词：

X-RAY CRYSTALLOGRAPHY; IMMUNOSUPPRESSIVE DRUG BINDING PROTEIN; PEPTIDYL-PROLYL ISOMERASE; CYCLOSPORINE-A;

D O I：

10.1073/pnas.88.21.9483

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The structure of the unligated human T-cell recombinant cyclophilin has been determined at 3 angstrom resolution by multiple isomorphous replacement methods and refined at 2.5 angstrom resolution to an R factor of 0.209. The root-mean-square errors of the bond lengths and bond angles are 0.013 angstrom and 2.8-degrees from ideal geometry, respectively. The overall structure is a beta-barrel, consisting of eight antiparallel beta-strands wrapping around the barrel surface and two alpha-helices sitting on the top and the bottom closing the barrel. Inside the barrel, seven aromatic and other hydrophobic residues form a compact hydrophobic core. A loop of Lys-118 to His-126 and four beta-strands (B3-B6) constitute a pocket we speculate to be the binding site of cyclosporin A.

引用

页码：9483 / 9487

页数：5

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