EVIDENCE FOR SPONTANEOUS CONVERSION OF MEX- TO MEX+ IN HUMAN LYMPHOBLASTOID-CELLS

被引：9

作者：

ARITA, I ^{[1
]}

FUJIMORI, A ^{[1
]}

TAKEBE, H ^{[1
]}

TATSUMI, K ^{[1
]}

机构：

[1] KYOTO UNIV,FAC MED,DEPT MOLEC ONCOL,KYOTO 606,JAPAN

来源：

CARCINOGENESIS | 1990年 / 11卷 / 10期

关键词：

D O I：

10.1093/carcin/11.10.1733

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A series of human lymphoblastoid cell lines (LCLs) called Mex- were defined by Sklar and Strauss on the basis of their inability to remove O6-methylguanine from DNA. Instability of Mex- has previously been shown as a population phenotype of LCLs. We examined whether Mex- as a cellular phenotype is spontaneously convertible or not. At the population doubling number (PDN) 23 after recloning, two out of 15 independent sibcultures derived from a Mex- LCL, AT1-1, were found to contain a small fraction of Mex+ cells after treatment with 1-(4-amino-2-methyl-5-pyr-imidinyl)methyl-3-(2-chloroethyl)-3-nitrosourea (ACNU). Three Mex+ subclones were identified without exposure to ACNU among 486 subclones from replica plating of an expanded Mex- clone (PDN30). The rate of spontaneous conversion was estimated to be in the range of 10-8-10-7 per cell per generation by the fluctuation analyses on two Mex- subclones. These results strongly support the hypothesis that Mex- as a cellular phenotype is spontaneously convertible to Mex+. © 1990 Oxford University Press.

引用

页码：1733 / 1738

页数：6

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