OLIGONUCLEOTIDE PROBE DETERMINATION OF TETRACYCLINE-RESISTANT BACTERIA ISOLATED FROM CATFISH PONDS

被引：16
作者：
DEPAOLA, A
HILL, WE
HARRELL, FM
机构：
[1] US FDA,SEAFOOD PROD RES CTR,BOTHELL,WA 98041
[2] US FDA,MIDWEST LAB MICROBIAL INVEST,MINNEAPOLIS,MN 55401
来源：
MOLECULAR AND CELLULAR PROBES | 1993年 / 7卷 / 05期
关键词：
OLIGONUCLEOTIDE PROBES; TETRACYCLINE-RESISTANT BACTERIA; CATFISH PONDS;
D O I：
10.1006/mcpr.1993.1051
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Oligonucleotide probes for class A, B, and C tetracycline resistance determinants were synthesized and tested individually and in combination for hybridization with Gram-negative isolates previously characterized by restriction fragment DNA probes. Similarly hybridization patterns were observed with either probe type. Gram-negative catfish pond bacteria were characterized by these oligonucleotide probes. The most common bacteria were Plesiomonas shigelloides (47.7%), Aeromonas hydrophila (28.8%) and Citrobacter freundii (13.3%). The class A (46.4%) tetracycline resistance determinant was more prevalent than B (19.8%) and C (0.3%). Some tetracycline-resistant isolates of each species, including most A. hydrophila (62.1%) isolates, failed to hybridize with any of the probes. © 1993 Academic Press, Limited.
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收藏
页码：345 / 348
页数：4
相关论文
共 10 条
[1]  
ATKINSON T, 1984, OLIGONUCLEOTIDE SYNT, P35
[2]   PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF TETRACYCLINE-RESISTANT AND OXYTETRACYCLINE-RESISTANT AEROMONAS-HYDROPHILA FROM CULTURED CHANNEL CATFISH (ICTALURUS-PUNCTATUS) AND THEIR ENVIRONMENTS [J].
DEPAOLA, A ;
FLYNN, PA ;
MCPHEARSON, RM ;
LEVY, SB .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (07) :1861-1863
[3]  
HILL WE, 1986, J ASSOC OFF ANA CHEM, V69, P531
[4]   NUCLEOTIDE-SEQUENCE OF THE TN10 ENCODED TETRACYCLINE RESISTANCE GENE [J].
HILLEN, W ;
SCHOLLMEIER, K .
NUCLEIC ACIDS RESEARCH, 1983, 11 (02) :525-539
[5]   TETRACYCLINE RESISTANCE GENES IN KENYAN HOSPITAL ISOLATES OF SALMONELLA-TYPHIMURIUM [J].
KARIUKI, S ;
MIRZA, NB ;
WASTESON, Y ;
SENERWA, D ;
GATHUMA, JM ;
OLSVIK, O .
APMIS, 1992, 100 (07) :629-634
[6]   FREQUENCY OF TETRACYCLINE RESISTANCE DETERMINANT CLASSES AMONG LACTOSE-FERMENTING COLIFORMS [J].
MARSHALL, B ;
TACHIBANA, C ;
LEVY, SB .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1983, 24 (06) :835-840
[7]   A NEW TETRACYCLINE-RESISTANCE DETERMINANT, CLASS-E, ISOLATED FROM ENTEROBACTERIACEAE [J].
MARSHALL, B ;
MORRISSEY, S ;
FLYNN, P ;
LEVY, SB .
GENE, 1986, 50 (1-3) :111-117
[8]   ANTIBIOTIC-RESISTANCE IN GRAM-NEGATIVE BACTERIA FROM CULTURED CATFISH AND AQUACULTURE PONDS [J].
MCPHEARSON, RM ;
DEPAOLA, A ;
ZYWNO, SR ;
MOTES, ML ;
GUARINO, AM .
AQUACULTURE, 1991, 99 (3-4) :203-211
[9]   REVISED SEQUENCE OF THE TETRACYCLINE-RESISTANCE GENE OF PBR322 [J].
PEDEN, KWC .
GENE, 1983, 22 (2-3) :277-280
[10]   THE TETRACYCLINE RESISTANCE DETERMINANTS OF RP1 AND TN1721 - NUCLEOTIDE-SEQUENCE ANALYSIS [J].
WATERS, SH ;
ROGOWSKY, P ;
GRINSTED, J ;
ALTENBUCHNER, J ;
SCHMITT, R .
NUCLEIC ACIDS RESEARCH, 1983, 11 (17) :6089-6105
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