A 300 KILOBASE INTERVAL GENETIC-MAP OF RICE INCLUDING 883 EXPRESSED SEQUENCES

被引：443

作者：

KURATA, N

NAGAMURA, Y

YAMAMOTO, K

HARUSHIMA, Y

SUE, N

WU, J

ANTONIO, BA

SHOMURA, A

SHIMIZU, T

LIN, SY

INOUE, T

FUKUDA, A

SHIMANO, T

KUBOKI, Y

TOYAMA, T

MIYAMOTO, Y

KIRIHARA, T

HAYASAKA, K

MIYAO, A

MONNA, L

ZHONG, HS

TAMURA, Y

WANG, ZX

MOMMA, T

UMEHARA, Y

YANO, M

SASAKI, T

MINOBE, Y

机构：

[1] AICHI KEN AGR RES CTR, NAGAKUTE, AICHI 48011, JAPAN

[2] WAKAYAMA AGR EXPT STN, WAKAYAMA 64004, JAPAN

[3] IBARAKI AGR CTR, INST PLANT BIOTECHNOL, IWAMA, IBARAKI 31902, JAPAN

[4] MIYAGI PREFECTURAL FURUKAWA AGR EXPT STN, FURUKAWA, MIYAGI, JAPAN

来源：

NATURE GENETICS | 1994年 / 8卷 / 04期

关键词：

D O I：

10.1038/ng1294-365

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have constructed a high resolution rice genetic map containing 1,383 DNA markers at an average interval of 300 kilobases (kb). The markers, distributed along 1,575 cM on 12 linkage groups, comprise 883 cDNAs, 265 genomic DNAs, 147 randomly amplified polymorphic DNAs (RAPD) and 88 other DNAs. cDNAs were derived from rice root and callus, analysed by single-run sequencing and searched for similarities with known proteins. Nearly 260 rice genes are newly identified and mapped, and genomic DNA and cloned RAPD fragments were also sequenced to generate STSs. Our map is the first significant gene expression map in plants. It is also the densest genetic map available in plants and the first to be backed up comprehensively by clone sequence data.

引用

页码：365 / 372

页数：8

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