GENETIC-TRANSFORMATION AND SOMATIC EMBRYOGENESIS IN LATHYRUS-SATIVUS

被引：12

作者：

BARNA, KS ^{[1
]}

MEHTA, SL ^{[1
]}

机构：

[1] INDIAN AGR RES INST,DIV BIOCHEM,NEW DELHI 110012,INDIA

来源：

JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY | 1995年 / 4卷 / 02期

关键词：

AGROBACTERIUM TUMEFACIENS; BIOLISTIC METHOD; GENETIC TRANSFORMATION; GRAIN LEGUME; GUS EXPRESSION; LATHYRUS SATIVUS;

D O I：

10.1007/BF03262955

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct somatic embryogenesis and genetic transformation has been achieved in Lathyrus sativus. The genetic transformation was achieved by Agrobacterium tumefaciens and biolistic methods of gene transfer. Somatic embryogenesis was obtained from immature leaflets and nodal segments by using 2,4-D in the induction medium. Somatic embryos formed germinated on growth regulator-free medium. The transgenic nature of the transformants was confirmed by GUS Expression assay. The transgenic shoots/plantlets were obtained only from tissue transformed with biolistic method of gene transfer.

引用

页码：67 / 71

页数：5

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