EFFICIENT AND GENTLE ELUTION OF ANTIBODIES FROM AN IMMOBILIZED POLYPEPTIDE ANTIGEN BY SATURATED MAGNESIUM-CHLORIDE

被引：3

作者：

BOYD, S

YAMAZAKI, H

机构：

[1] CARLETON UNIV,DEPT BIOL,OTTAWA K1S 5B6,ONTARIO,CANADA

[2] CARLETON UNIV,INST BIOCHEM,OTTAWA K1S 5B6,ONTARIO,CANADA

来源：

BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 11期

关键词：

D O I：

10.1007/BF00153754

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A model antigen, rabbit immunoglobulin G, was immobilized onto polyester cloth by adsorption. The antigen cloth was reacted with sheep anti-rabbit IgG antibody. Antibody bound to the antigen cloth was nearly quantitatively eluted by saturated MgCl2, whereas a commercial antibody eluent slowly eluted only about 70 % of the antibody. Exposure of antibody to saturated MgCl2 for 30 min resulted in no loss of immunoactivity. Saturated MgCl2, therefore, is an ideal eluent in immunoaffinity purification of antibodies.

引用

页码：827 / 832

页数：6