TRANSDUCING BACTERIOPHAGE-LAMBDA CARRYING STRUCTURAL GENE FOR ELONGATION FACTOR-TS

被引：28

作者：

FRIESEN, JD

PARKER, J

WATSON, RJ

BENDIAK, D

REEH, SV

PEDERSEN, S

FIIL, NP

机构：

[1] YORK UNIV, DEPT BIOL, DOWNSVIEW M3J 1P3, ONTARIO, CANADA

[2] UNIV COPENHAGEN, INST MICROBIOL, DK-1353 COPENHAGEN, DENMARK

来源：

MOLECULAR AND GENERAL GENETICS | 1976年 / 148卷 / 01期

关键词：

D O I：

10.1007/BF00268549

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A specialized transducing bacteriophage .lambda.dpolCdapD-9 was isolated that carries the structural gene for EF[elongation factor]-Ts (tsf). The presence of EF-Ts among the proteins synthesized under the direction of this phage in UV-inactivated cells [Escherichia coli] was detected by 2-dimensional gel electrophoresis and was verified by antibody precipitation. In an induced lysogen of this phage the relative rate of synthesis of EF-Ts is increased 4-fold. The structural genes for ribosomal protein S2 (rpsB) and RNA polymerase .sigma. factor (sit) probably also lie on .lambda.dpolCdapD-9.

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收藏

页码：93 / 98

页数：6

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