Development of multiplex-PCR assay for rapid detection of Candida spp

被引：1
|
作者：
Tarini, Ni Made A. [1 ]
Wahid, Mardiastuti H. [2 ]
Ibrahim, Fera [2 ]
Yasmon, Andi [2 ]
Djauzi, Samsuridjal [3 ]
机构：
[1] Univ Udayana, Fac Med, Dept Clin Microbiol, Bali, Indonesia
[2] Univ Indonesia, Dept Microbiol, Fac Med, Jakarta, Indonesia
[3] Univ Indonesia, Ciptomangunkusumo Hosp, Fac Med, Allerg Immunol Div,Dept Internal Med, Jakarta, Indonesia
来源：
MEDICAL JOURNAL OF INDONESIA | 2010年 / 19卷 / 02期
关键词：
Candida spp; multiplex-PCR;
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Aim Candida spp. infection commonly occur in immunocompromised patients. Biochemical assay for identifi cation of Candida spp. is time-consuming and shows many undetermined results. Specifi c detection for antibody, antigen and metabolites of Candida spp. had low sensitivity and specifi city. In this study, we developed a rapid diagnostic method, Multiplex-PCR, to identify Candida spp. Methods Five Candida spp. isolates were cultured, identifi ed with germ tube and API r 20 C AUX (BioMerieux r SA) kit. Furthermore, DNA was purifi ed by QIAamp DNA mini (Qiagen r) kit for Multiplex-PCR assay. Results DNA detection limit by Multiplex-PCR assays for C. albicans, C. tropicalis, C. parapsilosis, C. krusei and C. glabrata were 4 pg, 0,98 pg, 0,98 pg, 0,5 pg and 16 pg respectively. This assay was also more sensitive than culture in that Multiplex-PCR could detect 2.6-2.9 x 100 CFU/ml, whereas culture 2.6-2.9 x 102 CFU/ml. Conclusion Multiplex-PCR is much more sensitive than culture and thus, can be recommended as a sensitive and specifi c assay for identifi cation of Candida spp.
引用
收藏
页码：83 / 87
页数：5
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