PRODUCTION OF TRANSGENIC PEA (PISUM-SATIVUM L) PLANTS BY AGROBACTERIUM-TUMEFACIENS - MEDIATED GENE-TRANSFER

被引：86
作者：
PUONTIKAERLAS, J
ERIKSSON, T
ENGSTROM, P
机构：
[1] Department of Physiological Botany, University of Uppsala, Uppsala, S-751 21
来源：
THEORETICAL AND APPLIED GENETICS | 1990年 / 80卷 / 02期
关键词：
Agrobacterium tumefaciens; Pea; Pisum sativum; Regeneration; Transformation;
D O I：
10.1007/BF00224394
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
A transformation system that allows regeneration of transgenic pea plants from calli selected for antibiotic resistance was developed. Explants from axenic shoot cultures and seedling epicotyls were cocultivated with nononcogenic Agrobacterium tumefaciens strains, and transformed callus could be selected on callus-inducing media containing either 15 mg/l hygromycin or 75 mg/l kanamycin. After several passages on regeneration medium, shoot organogenesis could be reproducibly induced on hygromycin-resistant calli, but not on the calli selected for kanamycin resistance. Regenerated shoots could subsequently be rooted and transferred into the greenhouse. In addition, the effects of different callus-inducing and growth media on organogenesis were investigated. The transformation of the calli and regenerated plants was confirmed by DNA analysis. © 1990 Springer-Verlag.
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页码：246 / 252
页数：7
相关论文
共 33 条
[1]  
BEDBROOK J, 1981, PLANT MOL BIOL NEWSL, V2, P24
[2]   MORPHOGENETIC AND CELLULAR REORIENTATIONS INDUCED BY AGROBACTERIUM-RHIZOGENES (STRAIN-1855, STRAIN-2659 AND STRAIN-8196) ON CARROT, PEA AND TOBACCO [J].
BERCETCHE, J ;
CHRIQUI, D ;
ADAM, S ;
DAVID, C .
PLANT SCIENCE, 1987, 52 (03) :195-210
[3]  
DEBLAERE R, 1985, NUCLEIC ACIDS RES, V13, P4774
[4]   EVALUATION OF SELECTABLE MARKERS FOR RICE TRANSFORMATION [J].
DEKEYSER, R ;
CLAES, B ;
MARICHAL, M ;
VANMONTAGU, M ;
CAPLAN, A .
PLANT PHYSIOLOGY, 1989, 90 (01) :217-223
[5]   TRANSGENIC RYE PLANTS OBTAINED BY INJECTING DNA INTO YOUNG FLORAL TILLERS [J].
DELAPENA, A ;
LORZ, H ;
SCHELL, J .
NATURE, 1987, 325 (6101) :274-276
[6]  
Dellaporta S.L., 1983, PLANT MOL BIOL REP, V1, P19, DOI [10.1007/BF02712670, DOI 10.1007/BF02712670]
[7]   TRANSFORMATION OF BRASSICA-NAPUS WITH AGROBACTERIUM-TUMEFACIENS BASED VECTORS [J].
FRY, J ;
BARNASON, A ;
HORSCH, RB .
PLANT CELL REPORTS, 1987, 6 (05) :321-325
[8]   NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS [J].
GAMBORG, OL ;
MILLER, RA ;
OJIMA, K .
EXPERIMENTAL CELL RESEARCH, 1968, 50 (01) :151-+
[9]  
HAIN R, 1985, MOL GEN GENET, V199, P166
[10]  
HAMMATT N, 1986, CELL CULTURE SOMATIC, V3, P67
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