CA2+/CALMODULIN-DEPENDENT PROTEIN-PHOSPHORYLATION IS NOT ALTERED BY AMYGDALOID KINDLING

被引：4
作者：
BURDETTE, LJ [1 ]
OCALLAGHAN, JP [1 ]
机构：
[1] US EPA,DIV NEUROTOXICOL,RES TRIANGLE PK,NC 27711
来源：
BRAIN RESEARCH BULLETIN | 1991年 / 26卷 / 03期
关键词：
KINDLING; SEIZURES; AMYGDALA; HIPPOCAMPUS; CALMODULIN; PHOSPHORYLATION;
D O I：
10.1016/0361-9230(91)90024-E
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Kindling is a process in which episodic electrical stimulation permanently increases seizure susceptibility. One mechanism to account for a change in seizure susceptibility is some alteration in signal transduction, possibly at the level of second messenger systems. In this study, male Long-Evans rats were kindled in the amygdala, and Ca2+/calmodulin (Ca2+/CaM)-dependent protein phosphorylation was assessed at the site of the primary kindled focus using one- and two-dimensional gel electrophoresis. In vitro phosphorylation of membrane and cytosol fractions in the presence or absence of Ca2+/CaM did not differentiate kindled from nonkindled amygdaloid tissue. These results suggest that changes in Ca2+/CaM-dependent phosphorylation are not related to the mechanism(s) underlying the establishment of an amygdaloid kindled focus.
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收藏
页码：455 / 459
页数：5
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