A STUDY OF OLIGONUCLEOTIDE REASSOCIATION USING LARGE ARRAYS OF OLIGONUCLEOTIDES SYNTHESIZED ON A GLASS SUPPORT

被引：79
作者：
MASKOS, U [1 ]
SOUTHERN, EM [1 ]
机构：
[1] UNIV OXFORD, DEPT BIOCHEM, OXFORD OX1 3QU, ENGLAND
来源：
NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 20期
关键词：
D O I：
10.1093/nar/21.20.4663
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An extensive analysis of oligonucleotide interactions was carried out by hybridising a synthetic pool of 256 10mers, A(CT)8A, representing all oligopyrimidine octamer sequences to an array of four copies of all 256 different octapurine sequences. The resulting 256 duplexes were quantified by phosphorimaging and analysed to determine the dependence of duplex formation on base composition, sequence, and salt concentration. The results show that the base composition dependence of duplex formation can be reduced by high concentrations of tetramethylammonium chloride. This chaotropic solvent also increases duplex yield by up to fiftyfold.
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页码：4663 / 4669
页数：7
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