RAPID AND EFFICIENT SITE-SPECIFIC MUTAGENESIS WITHOUT PHENOTYPIC SELECTION

被引：6481

作者：

KUNKEL, TA

机构：

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1985年 / 82卷 / 02期

关键词：

D O I：

10.1073/pnas.82.2.488

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

引用

页码：488 / 492

页数：5

共 22 条

[1] RESTORATION OF THE BIOLOGICAL-ACTIVITY OF INVITRO SYNTHESIZED PHI-X DNA BY TRANSFECTION OF UNG- SPHEROPLASTS OR DUTPASE TREATMENT [J].

BAAS, PD ;

VANTEEFFELEN, HAAM ;

TEERTSTRA, WR ;

JANSZ, HS ;

VEENEMAN, GH ;

VANDERMAREL, GA ;

VANBOOM, JH .

FEBS LETTERS, 1980, 110 (01) :15-20

[2] SITE-SPECIFIC MUTAGENESIS USING OLIGODEOXYRIBONUCLEOTIDES - ISOLATION OF A PHENOTYPICALLY SILENT PHI-CHI-174 MUTANT, WITH A SPECIFIC NUCLEOTIDE DELETION, AT VERY HIGH-EFFICIENCY [J].

GILLAM, S ;

ASTELL, CR ;

SMITH, M .

GENE, 1980, 12 (1-2) :129-137

[3] SITE-SPECIFIC MUTAGENESIS USING SYNTHETIC OLIGODEOXYRIBONUCLEOTIDE PRIMERS .2. INVITRO SELECTION OF MUTANT-DNA [J].

GILLAM, S ;

SMITH, M .

GENE, 1979, 8 (01) :99-106

[4] ESCHERICHIA-COLI MUTATOR MUTANTS DEFICIENT IN METHYLATION-INSTRUCTED DNA MISMATCH CORRECTION [J].

GLICKMAN, BW ;

RADMAN, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (02) :1063-1067

[5] DIRECTED MUTAGENESIS OF DNA CLONED IN FILAMENTOUS PHAGE - INFLUENCE OF HEMIMETHYLATED GATC SITES ON MARKER RECOVERY FROM RESTRICTION FRAGMENTS [J].

KRAMER, W ;

SCHUGHART, K ;

FRITZ, HJ .

NUCLEIC ACIDS RESEARCH, 1982, 10 (20) :6475-6485

[6] MUTATIONAL SPECIFICITY OF DEPURINATION [J].

KUNKEL, TA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (05) :1494-1498

[7]

LATHE R, 1983, GENETIC ENG, V4, P1

[8]

Lindahl T, 1979, Prog Nucleic Acid Res Mol Biol, V22, P135

[9] FILAMENTOUS COLIPHAGE M13 AS A CLONING VEHICLE - INSERTION OF A HINDII FRAGMENT OF LAC REGULATORY REGION IN M13 REPLICATIVE FORM INVITRO - (SINGLE-STRANDED-DNA PHAGE-BLUNT END LIGATION LACTOSE OPERON ALPHA-COMPLEMENTATION) [J].

MESSING, J ;

GRONENBORN, B ;

MULLERHILL, B ;

HOFSCHNEIDER, PH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (09) :3642-3646

[10] INSERTION OF NUCLEOTIDES OPPOSITE APURINIC APYRIMIDINIC SITES IN DEOXYRIBONUCLEIC-ACID DURING INVITRO SYNTHESIS - UNIQUENESS OF ADENINE-NUCLEOTIDES [J].

SAGHER, D ;

STRAUSS, B .

BIOCHEMISTRY, 1983, 22 (19) :4518-4526

← 1 2 3 →