CORTICOTROPIN-RELEASING FACTOR IS SECRETED IN THE BE(2)-C NEUROBLASTOMA CELL AND IS RESPONSIVE TO FORSKOLIN

被引：4
作者：
KASCKOW, JW
OWENS, MJ
STIPETIC, MD
HAN, JH
NEMEROFF, CB
机构：
来源：
LIFE SCIENCES | 1994年 / 54卷 / 23期
关键词：
CORTICOTROPIN-RELEASING FACTOR; NEUROBLASTOMA CELLS; RADIOIMMUNOASSAY; FORSKOLIN;
D O I：
10.1016/0024-3205(94)90121-X
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The BE(2)-C human neuroblastoma has been shown to express corticotropin-releasing factor (CRF) messenger RNA. In this study, BE(2)-C cells were treated 5 days with 5 mu M retinoic acid. Cell extracts were also applied onto a C18 Vydac column and fractions were assayed for CRF-like immunoreactivity (CRF-LI) which coincided in time of elution with oxidized or non-oxidized CRF synthetic CRF standard. With forskolin treatment, secretion media and cell extract CRF-LI increased in a concentration-dependent fashion. Thus, this cell line synthesizes and secretes CRF and is a good model for studying CRF regulation.
引用
收藏
页码：1825 / 1829
页数：5
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