AUTOANTIBODIES TO HUMAN SPERM BASIC NUCLEAR PROTEINS IN INFERTILE AND VASECTOMIZED MEN - CHARACTERIZATION OF ANTIGENS AND EPITOPES RECOGNIZED BY ANTIBODIES

被引:9
作者
ROUSSEAUXPREVOST, R
DEALMEIDA, M
JOUANNET, P
HUBLAU, P
SAUTIERE, P
ROUSSEAUX, J
机构
[1] CHU BICETRE,HISTOL EMBRYOL CYTOGENET LAB,F-94270 LE KREMLIN BICETR,FRANCE
[2] FAC PHARM LILLE,CHIM ORGAN LAB,F-59045 LILLE,FRANCE
关键词
D O I
10.1016/0161-5890(92)90127-J
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The sera of vasectomized men and of patients with immune infertility were used to study the antigens and epitopes of sperm nuclear proteins that bind antibodies in these sera. No reaction with sperm histones was observed except for one serum. P1, P2 protamines and pro-P2 protamines were recognized by auto-antibodies. Studies with peptides derived from P1 and P2 protamines and with mammalian protamines related to HPI showed that antibodies are mainly specific for a folded protamine molecule, more especially antibodies from vasectomized men. These results disagree with the random coil model proposed for protamines by several previous works. A cross-reactivity between P1 and P2 protamines was observed only for the whole molecules and not for peptides derived from them. This observation suggests that the two classes of protamines, different in sequence, may have a similar folding and thereby may be functionally equivalent.
引用
收藏
页码:895 / 902
页数:8
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