ISOLATION AND IN-VITRO CULTIVATION OF AN UNIDENTIFIED CILIATE CAUSING SCUTICOCILIATOSIS IN JAPANESE FLOUNDER (PARALICHTHYS-OLIVACEUS)

被引：58

作者：

YOSHINAGA, T

NAKAZOE, J

机构：

来源：

FISH PATHOLOGY | 1993年 / 28卷 / 03期

关键词：

D O I：

10.3147/jsfp.28.131

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The ciliate causing scuticociliatosis in Japanese flounder was isolated, and axenic cultures were established. Sterilization of the ciliate was accomplished by serial washing with capillary pipettes. Growth of the ciliate was compared in different dilutions of Millport S solution containing 2% proteose peptone and 1% yeast extract at different temperatures. Optimum growth was obtained at dilution of 1:2 (osmolarity: 438 mosm) at 22.5-27.5-degrees-C with a maximum cell density of 1.0 X 10(4) cells/ml.

引用

页码：131 / 134

页数：4

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[No title captured]

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