MONOCLONAL-ANTIBODIES WITH SELECTIVE SPECIFICITY FOR ALZHEIMER TAU ARE DIRECTED AGAINST PHOSPHATASE-SENSITIVE EPITOPES

被引：316

作者：

MERCKEN, M

VANDERMEEREN, M

LUBKE, U

SIX, J

BOONS, J

VANDEVOORDE, A

MARTIN, JJ

GHEUENS, J

机构：

[1] INNOGENET,IND PK ZWIJNAARDE 7,B-9052 GENT,BELGIUM

[2] UNIV INSTELLING ANTWERP,BORN BUNGE FDN,NEUROBIOL LABS,B-2610 WILRIJK,BELGIUM

[3] UNIV INSTELLING ANTWERP,BORN BUNGE FDN,NEUROPATHOL LABS,B-2610 WILRIJK,BELGIUM

来源：

ACTA NEUROPATHOLOGICA | 1992年 / 84卷 / 03期

关键词：

ALZHEIMERS DISEASE; TAU; PHOSPHORYLATION; MONOCLONAL ANTIBODIES;

D O I：

10.1007/BF00227819

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

A modified form of the microtubule-associated protein Tau is the major component of the paired helical filaments (PHF) found in Alzheimer's disease. The characterization of these posttranslational Tau modifications is hindered by the lack of sufficient PHF-Tau-specific markers. Here we describe several monoclonal antibodies, prepared by immunization with PHF, two of which showed a selective specificity for PHF-Tau without cross-reactivity with normal Tau. Epitope recognition by these two monoclonals was sensitive to alkaline phosphatase treatment. In Western blotting these monoclonal antibodies reacted specifically with the abnormally phosphorylated epitopes on Alzheimer's disease-associated PHF-Tau. One of the new antibodies can be used for the construction of a sandwich enzyme-linked immunosorbent assay for the specific detection of PHF-Tau without cross-reactivity to normal Tau proteins.

引用

页码：265 / 272

页数：8

共 33 条

[1]

BINDER LI, 1985, J CELL BIOL, V101, P1371, DOI 10.1083/jcb.101.4.1371

[2] TAU IN ALZHEIMER NEUROFIBRILLARY TANGLES - N-TERMINAL AND C-TERMINAL REGIONS ARE DIFFERENTIALLY ASSOCIATED WITH PAIRED HELICAL FILAMENTS AND THE LOCATION OF A PUTATIVE ABNORMAL PHOSPHORYLATION SITE [J].