IMMOBILIZATION OF GLUCOSE-OXIDASE - A COMPARISON OF ENTRAPMENT AND COVALENT BONDING

被引:66
作者
ARICA, MY [1 ]
HASIRCI, V [1 ]
机构
[1] MIDDLE E TECH UNIV,DEPT BIOL SCI,BIOTECHNOL RES UNIT,06531 ANKARA,TURKEY
关键词
ENTRAPMENT; COVALENT BONDING; PHEMA; GLUCOSE OXIDASE;
D O I
10.1002/jctb.280580313
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Glucose oxidase was immobilized onto poly(2-hydroxyethyl methacrylate) (pHEMA) membranes by two methods: by covalent bonding through epichlorohydrin and by entrapment between pHEMA membranes. The highest immobilization efficiency was found to be 17.4% and 93.7% for the covalent bonding and entrapment, respectively. The K(m) values were 5.9 mmol dm-3, 8.8 mmol dm-3 and 12.4 mmol dm-3 for free, bound and entrapped enzyme, respectively. The V(max) values were 0.071 mmol dm-3 min-1, 0.067 mmol dm-3 min-1 and 0.056 mmol dm-3 min-1 for free, bound and entrapped enzyme. When the medium was saturated with oxygen, K(m) was not significantly altered but V(max) was. The optimum pH values for the free, covalently-bound and entrapped enzyme were determined to be 5, 6, and 7, respectively. The optimum temperature was 30-degrees-C for free or covalently-bound enzyme but 35-degrees-C for entrapped enzyme. The deactivation constant for bound enzyme was determined as 1.7 x 10(-4) min-1 and 6.9 x 10(-4) min-1 for the entrapped enzyme.
引用
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页码:287 / 292
页数:6
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