ELECTRON-IMPACT IONIZATION DETECTION OF SCOPOLAMINE BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY IN RAT PLASMA AND BRAIN

被引：16

作者：

DEUTSCH, J

SONCRANT, TT

GREIG, NH

RAPOPORT, SI

机构：

[1] Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Bethesda

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1990年 / 528卷 / 02期

关键词：

D O I：

10.1016/S0378-4347(00)82391-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Scopolamine, a muscarinic receptor antagonist, was measured in rat plasma and brain using gas chromatography-mass spectrometry with electron-impact ionization detection. Extracted scopolamine was either directly derivatized or first hydrolyzed to scopine, then derivatized with heptafluorobutyric anhydride, separated on a capillary column and quantified by mass fragmentography. Electron-impact ionization produced a common fragment peak at m z 138 that was monitored along with trideuterated scopolamine, an internal standard ( m z 141). The method can be used to measure scopolamine concentrations of 2 ng/ml in rat plasma and 20 ng/g in rat brain. © 1990.

引用

页码：325 / 331

页数：7

共 6 条

[1] EVIDENCE FOR A DIRECT CHOLINERGIC INVOLVEMENT IN THE SCOPOLAMINE-INDUCED AMNESIA IN MONKEYS - EFFECTS OF CONCURRENT ADMINISTRATION OF PHYSOSTIGMINE AND METHYLPHENIDATE WITH SCOPOLAMINE [J].