ANALYSIS OF THE TDH GENE CLONED FROM A TDH GENE-POSITIVE AND TRH GENE-POSITIVE STRAIN OF VIBRIO-PARAHAEMOLYTICUS

被引：25

作者：

BABA, K ^{[1
]}

SHIRAI, H ^{[1
]}

TERAI, A ^{[1
]}

TAKEDA, Y ^{[1
]}

NISHIBUCHI, M ^{[1
]}

机构：

[1] KYOTO UNIV,FAC MED,DEPT MICROBIOL,SAKYO KU,KYOTO 606,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1991年 / 35卷 / 03期

关键词：

D O I：

10.1111/j.1348-0421.1991.tb01554.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A variant of the gene (tdh) encoding thermostable direct hemolysin (TDH) was cloned from the chromosome of Vibrio parahaemolyticus AQ3860, which gave positive results in the hybridization tests with the tdh gene probe and the trh (tdh-related hemolysin) gene probe and showed a low level of reaction in an enzyme-linked immunosorbent assay for TDH. Nucleotide sequence analysis of the cloned gene (tdh5) provided no evidence that tdh5 is evolutionally closer to the trh gene than the other tdh genes. The tdh5 gene was flanked by 40 base-pair sequences constituting perfect inverted repeats, which may suggest association of the tdh5 gene with insertion sequence-like structure. These results suggest that the tdh5 gene and the trh gene were not originally produced by gene duplication in AQ3860 but rather that one of the two genes moved into AQ3860 from an external source.

引用

页码：253 / 258

页数：6

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