OXIDATIVE-PHOSPHORYLATION BY MITOCHONDRIA EXTRACTED FROM DRY SUNFLOWER SEEDS

被引:53
作者
ATTUCCI, S
CARDE, JP
RAYMOND, P
SAINTGES, V
SPITERI, A
PRADET, A
机构
[1] INRA,CR BORDEAUX,PHYSIOL VEGETALE STN,BP 81,F-33883 VILLENAVE DORNON,FRANCE
[2] UNIV BORDEAUX 1,F-33405 TALENCE,FRANCE
关键词
D O I
10.1104/pp.95.2.390
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The role of mitochondria in the phosphorylation of ADP to ATP in the early steps of seed germination has been studied. Mitochondria were extracted from dry sunflower (Helianthus annuus) seeds. Adenylate kinase-dependent ATP synthesis was inhibited by p1,p5-di(adenosine-5')pentaphosphate. Synthesis of ATP was observed with the different substrates: citrate, alpha-ketoglutarate, succinate, malate, pyruvate or NADH. This synthesis was activated by cytochrome c, and inhibited by cyanide, oligomycin, carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone, and carboxyatractyloside. The ATP/O values with succinate were 0.85 and 1.2 in the absence or presence, respectively, of cytochrome c. Electron micrographs showed that mitochondria of dry tissues have different structures when observed in situ or in vitro after aqueous extraction, suggesting that profound changes occurred after the contact with the aqueous medium. These results confirm previous data obtained in vivo showing that mitochondria present in dry seeds are able to synthesize ATP as soon as the seeds are rehydrated.
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页码:390 / 398
页数:9
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