CLONING AND SEQUENCING OF A UNIQUE ANTIGEN MPT70 FROM MYCOBACTERIUM-TUBERCULOSIS H37RV AND EXPRESSION IN BCG USING ESCHERICHIA-COLI MYCOBACTERIA SHUTTLE VECTOR

被引：40

作者：

MATSUMOTO, S ^{[1
]}

MATSUO, T ^{[1
]}

OHARA, N ^{[1
]}

HOTOKEZAKA, H ^{[1
]}

NAITO, M ^{[1
]}

MINAMI, J ^{[1
]}

YAMADA, T ^{[1
]}

机构：

[1] NAGASAKI UNIV, SCH DENT, NAGASAKI 852, JAPAN

来源：

SCANDINAVIAN JOURNAL OF IMMUNOLOGY | 1995年 / 41卷 / 03期

关键词：

D O I：

10.1111/j.1365-3083.1995.tb03565.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

MPB70 is known to be an immunogenic mycobacterial protein secreted in large amounts from Mycobacterium bovis BCG (BCG) Tokyo, The analogous gene for MPT70 was cloned from Mycobacterium tuberculosis H37Rv which produces this protein in only a small amount. The gene encoding 193 amino acid residues including 30 amino acids for the signal peptide, the promoter-like sequence, and the ribosome-binding site, was completely identical to that of BCG Tokyo, Computer analysis revealed that the carboxy-terminal half of MPT70 was homologous to amino acid sequences of fasciclin I, osteoblast-specific factor 2 (OSF-2), and human transforming growth factor-beta induced gene product (beta IG-H3). Escherichia coli (E. coli) -mycobacteria shuttle vectors containing mpt70 or mpb70 genes 0.7kbp upstream of the 5' end of them were able to be expressed in BCG Pasteur which is a MPB70 low-producer, but the extent of the expression was not that of a high-producer.

引用

页码：281 / 287

页数：7

共 50 条

[11]

DAVID H, 1978, TUBERCLE, V59, P139

[12]

DEWIT L, 1990, NUCLEIC ACIDS RES, V18, P3995, DOI 10.1093/nar/18.13.3995

[13]

DYSON HJ, 1985, NATURE, V318, P480

[14] RHO-INDEPENDENT TERMINATION - DYAD SYMMETRY IN DNA CAUSES RNA-POLYMERASE TO PAUSE DURING TRANSCRIPTION INVITRO [J].