CHARACTERIZATION OF THE PNT1 PENTAMIDINE RESISTANCE GENE OF SACCHAROMYCES-CEREVISIAE

被引：16

作者：

LUDEWIG, G ^{[1
]}

STABEN, C ^{[1
]}

机构：

[1] UNIV KENTUCKY, TH MORGAN SCH BIOL SCI, LEXINGTON, KY 40506 USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1994年 / 38卷 / 12期

关键词：

D O I：

10.1128/AAC.38.12.2850

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Saccharomyces cerevisiae PNT1 gene was isolated and characterized. When present in high copy number in S. cerevisiae, PNT1 confers resistance to the anti-Pneumocystis carinii drug pentamidine. The PNT1 gene encodes a previously uncharacterized polypeptide of 409 amino acids. The predicted gene product is a very basic (pI 9.9) polypeptide with one potential membrane-associated region. PNT1 is located on chromosome XVR of S. cerevisiae. It is transcribed at a very low level. Overexpression of the gene increases resistance to the cytostatic and mitochondrial DNA-damaging effects of pentamidine and related cationic compounds. Disruption of the gene leads to slightly increased levels of susceptibility to pentamidine and some related compounds.

引用

页码：2850 / 2856

页数：7

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