AMINO-TERMINAL DOMAIN OF NF1 BINDS TO DNA AS A DIMER AND ACTIVATES ADENOVIRUS DNA-REPLICATION

被引:140
作者
GOUNARI, F [1 ]
DEFRANCESCO, R [1 ]
SCHMITT, J [1 ]
VANDERVLIET, PC [1 ]
CORTESE, R [1 ]
STUNNENBERG, H [1 ]
机构
[1] STATE UNIV UTRECHT,PHYSIOL CHEM LAB,3521 GD UTRECHT,NETHERLANDS
关键词
Adenovirus; DNA replication; NF1; oligomerization;
D O I
10.1002/j.1460-2075.1990.tb08143.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
NF1 is a DNA-binding protein involved in initiation of adenovirus DNA replication as well as in modulating the rate of transcription initiation of genes containing the sequence TGGCA. We show here that recombinant NF1 expressed via vaccina virus is transported into the nucleus and binds to its cognate sequences with the same specificity as NF1 purified from HeLa cells. Furthermore, the recombinant NF1 forms oligomers in solution and binds as a dimer to palindromic as well as half-site sequences. NF1 expressed via vaccina virus stimulates the initiation of adenovirus replication in vitro. The N-terminal 240 amino acids of the protein are sufficient for full DNA-binding activity as well as stimulation of adenovirus replication. By analysis of several NF1 mutants translated in vitro, we also define the minimal DNA-binding domain and localize the region responsible for DNA binding on the N-terminal and for oligomerization on the C-terminal side of this domain.
引用
收藏
页码:559 / 566
页数:8
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