The use of FTIR spectroscopy to monitor modifications in plant cell wall architecture caused by cellulose biosynthesis inhibitors

被引:103
作者
Alonso-Simon, Ana [1 ]
Garcia-Angulo, Penelope [1 ]
Melida, Hugo [1 ]
Encina, Antonio [1 ]
Alvarez, Jesus M. [1 ]
Acebes, Jose L. [1 ]
机构
[1] Univ Leon, Area Fisiol Vegetal, Fac CC Biol & Ambientales, Leon, Spain
关键词
FTIR; cellulose biosynthesis inhibitor; habituation/dehabituation;
D O I
10.4161/psb.6.8.15793
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fourier Transform InfraRed (FTIR) spectroscopy is a powerful and rapid technique for analyzing cell wall components and putative cross-links, which is able to non-destructively recognize polymers and functional groups and provide abundant information about their in muro organization. FTIR spectroscopy has been reported to be a useful tool for monitoring cell wall changes occurring in muro as a result of various factors, such as growth and development processes, mutations or biotic and abiotic stresses. This mini-review examines the use of FTIR spectroscopy in conjunction with multivariate analyses to monitor cell wall changes related to (1) the exposure of diverse plant materials to cellulose biosynthesis inhibitors (CBIs) and (2) the habituation/dehabituation of plant cell cultures to this kind of herbicides. The spectra analyses show differences not only regarding the inhibitor, but also regarding how long cells have been growing in its presence.
引用
收藏
页码:1104 / 1110
页数:7
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