ELECTROCHEMICAL ENZYME-IMMUNOASSAY

被引：7

作者：

HALSALL, HB

HEINEMAN, WR

JENKINS, SH

WEHMEYER, KR

DOYLE, MJ

WRIGHT, DS

机构：

[1] UNIV CINCINNATI, DEPT BIOCHEM, CINCINNATI, OH 45221 USA

[2] UNIV CINCINNATI, DEPT CHEM, BIOMED CHEM RES CTR, CINCINNATI, OH 45221 USA

来源：

JOURNAL OF RESEARCH OF THE NATIONAL BUREAU OF STANDARDS | 1988年 / 93卷 / 03期

关键词：

Biochemistry--Trace Analysis - Immunology--Antigen-Antibody Reactions - Trace Analysis;

D O I：

10.6028/jres.093.131

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

'Immunoassay' defines the body of techniques which use the antibody Ab) macromolecule, usually of the IgG class, for the detection and quantitation of an enormous range of simple and complex antigen (Ag) molecules. The success of the methods relies on both the specificity and formation constant of Ab used, and the ability to detect the interaction between Ab and Ag. Electrochemically based immunoassays at very low detection limits have also used enzyme amplification and ELISA, with either NAD/NADH and glucose-6-phosphate dehydrogenase, or phenyl phosphate/phenol and alkaline phosphatase. The greatest sensitivity thus far has been obtained with ELISA coupled with liquid chromatography with electrochemical detection (LCED). The assay is essentially a 'sandwich' ELISA, using an alkaline phosphatase labelled second Ab for amplification.

引用

页码：491 / 493

页数：3