DETECTION OF A PRFA-INDEPENDENT PROMOTER RESPONSIBLE FOR LISTERIOLYSIN GENE-EXPRESSION IN MUTANT LISTERIA-MONOCYTOGENES STRAINS LACKING THE PRFA REGULATOR

被引：41
作者：
DOMANN, E
WEHLAND, J
NIEBUHR, K
HAFFNER, C
LEIMEISTERWACHTER, M
CHAKRABORTY, T
机构：
[1] UNIV GIESSEN KLINIKUM,INST MED MIKROBIOL,W-6300 GIESSEN,GERMANY
[2] GESELL BIOTECHNOL FORSCH MBH,MIKROBIOL ABT,W-3300 BRAUNSCHWEIG,GERMANY
[3] UNIV WURZBURG,MED KLIN,INST KLIN CHEM & PATHOCHEM,W-8700 WURZBURG,GERMANY
[4] INST VIRION GMBH,W-8700 WURZBURG,GERMANY
来源：
INFECTION AND IMMUNITY | 1993年 / 61卷 / 07期
关键词：
D O I：
10.1128/IAI.61.7.3073-3075.1993
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Expression of listeriolysin, a major virulence factor of pathogenic Listeria monocytogenes, is positively regulated by the pleiotropic virulence regulator PrfA. In this study, we demonstrate that L. monocytogenes strains lacking the prfA regulator gene produce listeriolysin in small, albeit detectable, amounts when analyzed in a hemolysin assay and by immunoblots with listeriolysin-specific monoclonal antibodies. Transcriptional analysis revealed the existence of a PrfA-independent promoter that was responsible for the hemolytic activity expressed by these strains.
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页码：3073 / 3075
页数：3
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