CHARACTERIZATION OF HEPATOCYTE GROWTH-FACTOR EXPRESSED BY BACULOVIRUS

被引：11
作者：
LEE, HS
HUANG, GT
SHEU, JC
CHIOU, LL
LAI, MY
CHEN, DS
LEE, SC
机构：
[1] NATL TAIWAN UNIV,INST MOLEC MED,TAIPEI,TAIWAN
[2] NATL TAIWAN UNIV,DEPT INTERNAL MED,TAIPEI,TAIWAN
[3] NATL TAIWAN UNIV,DEPT EMERGENCY MED,TAIPEI,TAIWAN
[4] NATL TAIWAN UNIV,HEPATITIS RES CTR,TAIPEI,TAIWAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 197卷 / 02期
关键词：
D O I：
10.1006/bbrc.1993.2520
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To express recombinant hepatocyte growth factor (HGF), we cloned the cDNA by polymerase chain reaction. Functional recombinant HGF was expressed in insect cells by a baculovirus expression system. The recombinant HGF is a mixture of a single-chain precursor and a two-chain heterodimer. Under non-reducing conditions, they migrate together as a single band with MW 67 kDa on Western blots. Under reducing conditions, the precursor migrates at 91 kDa, α-chain at 64 kDa and β-chain at 34 kDa. About 25% of the expressed HGF is in the expression medium, which does have scatter activity on HepG2 and MDCK cells and proliferation activity on BN CL.2 cells (a mouse hepatocytes cell line) in a dose-dependent manner. © 1993 Academic Press. All rights reserved.
引用
收藏
页码：591 / 598
页数：8
相关论文
共 26 条
[1]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[2]   PURIFICATION AND PARTIAL CHARACTERIZATION OF HEPATOCYTE GROWTH-FACTOR FROM PLASMA OF A PATIENT WITH FULMINANT HEPATIC-FAILURE [J].
GOHDA, E ;
TSUBOUCHI, H ;
NAKAYAMA, H ;
HIRONO, S ;
SAKIYAMA, O ;
TAKAHASHI, K ;
MIYAZAKI, H ;
HASHIMOTO, S ;
DAIKUHARA, Y .
JOURNAL OF CLINICAL INVESTIGATION, 1988, 81 (02) :414-419
[3]   SCATTER FACTOR INDUCES BLOOD-VESSEL FORMATION INVIVO [J].
GRANT, DS ;
KLEINMAN, HK ;
GOLDBERG, ID ;
BHARGAVA, MM ;
NICKOLOFF, BJ ;
KINSELLA, JL ;
POLVERINI, P ;
ROSEN, EM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (05) :1937-1941
[4]   CHARACTERIZATION OF THE EFFECTS OF HUMAN PLACENTAL HGF ON RAT HEPATOCYTES [J].
HERNANDEZ, J ;
ZARNEGAR, R ;
MICHALOPOULOS, GK .
JOURNAL OF CELLULAR PHYSIOLOGY, 1992, 150 (01) :116-121
[5]   HEPATOCYTE GROWTH-FACTOR IS A POTENT MITOGEN FOR CULTURED RABBIT RENAL TUBULAR EPITHELIAL-CELLS [J].
IGAWA, T ;
KANDA, S ;
KANETAKE, H ;
SAITOH, Y ;
ICHIHARA, A ;
TOMITA, Y ;
NAKAMURA, T .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 174 (02) :831-838
[6]   DIRECT EVIDENCE THAT HEPATOCYTE GROWTH-FACTOR IS A HEPATOTROPHIC FACTOR FOR LIVER-REGENERATION AND HAS A POTENT ANTIHEPATITIS EFFECT INVIVO [J].
ISHIKI, Y ;
OHNISHI, H ;
MUTO, Y ;
MATSUMOTO, K ;
NAKAMURA, T .
HEPATOLOGY, 1992, 16 (05) :1227-1235
[7]   HEPATOCYTE GROWTH-FACTOR HEPATOPOIETIN-A STIMULATES THE GROWTH OF RAT-KIDNEY PROXIMAL TUBULE EPITHELIAL-CELLS (RPTE), RAT NONPARENCHYMAL LIVER-CELLS, HUMAN-MELANOMA CELLS, MOUSE KERATINOCYTES AND STIMULATES ANCHORAGE-INDEPENDENT GROWTH OF SV40-TRANSFORMED RPTE [J].
KAN, M ;
ZHANG, GH ;
ZARNEGAR, R ;
MICHALOPOULOS, G ;
MYOKEN, Y ;
MCKEEHAN, WL ;
STEVENS, JL .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 174 (01) :331-337
[8]   MARKED INCREASE OF HGF MESSENGER-RNA IN NON-PARENCHYMAL LIVER-CELLS OF RATS TREATED WITH HEPATOTOXINS [J].
KINOSHITA, T ;
TASHIRO, K ;
NAKAMURA, T .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 165 (03) :1229-1234
[9]   INDUCTION OF LIVER ALPHA-1 ACID GLYCOPROTEIN GENE-EXPRESSION INVOLVES BOTH POSITIVE AND NEGATIVE TRANSCRIPTION FACTORS [J].
LEE, YM ;
TSAI, WH ;
LAI, MY ;
CHEN, DS ;
LEE, SC .
MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (01) :432-442
[10]   HEPATOCYTE GROWTH-FACTOR (HEPATOPOIETIN-A) RAPIDLY INCREASES IN PLASMA BEFORE DNA-SYNTHESIS AND LIVER-REGENERATION STIMULATED BY PARTIAL-HEPATECTOMY AND CARBON-TETRACHLORIDE ADMINISTRATION [J].
LINDROOS, PM ;
ZARNEGAR, R ;
MICHALOPOULOS, GK .
HEPATOLOGY, 1991, 13 (04) :743-750
123