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DETERMINATION OF PALATINOSE IN FOOD BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH A POLARIZED PHOTOMETRIC DETECTOR
被引：0
作者：
YAMAMOTO, A
MATSUNAGA, A
MIZUKAMI, E
机构：
来源：
JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN
|
1992年
/
33卷
/
03期
关键词：
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;
POLARIZED PHOTOMETRIC DETECTION;
PALATINOSE;
SUCROSE;
D O I：
暂无
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Palatinose has attracted interest recently as a saccharide not inducing dental carries. Polarized photometric detection (PPD) is feasible by incorporating two polarizers into a conventional photometric detector, thus enabling the determination of optically active compounds such as palatinose in combination with high performance liquid chromatography (HPLC). A PPD system with a borate-form ligand-exchange column was highly specific for disaccharides. This specificity made the sample pretreatment easy. The extract from food was ultrafiltered through a Tosoh ULTRACENT-10 cartridge prior to HPLC injection. The lowest detection limit of palatinose was 0.03-mu-mol.
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页码：301 / 304
页数：4
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