EFFECTS OF 6-(10-HYDROXYDECYL)-2,3-DIMETHOXY-5-METHYL-1,4-BENZOQUINONE (IDEBENONE) AND RELATED BENZOQUINONES ON PORCINE PANCREAS PHOSPHOLIPASE A(2) ACTIVITY

被引：0
作者：
AMANO, T [1 ]
TERAO, S [1 ]
IMADA, I [1 ]
机构：
[1] TAKEDA CHEM IND LTD, DIV RES & DEV, YODOGAWA KU, OSAKA 532, JAPAN
来源：
BIOLOGICAL & PHARMACEUTICAL BULLETIN | 1995年 / 18卷 / 05期
关键词：
IDEBENONE; MEMBRANE; PHOSPHOLIPASE A(2); HPLC; BENZOQUINONE;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
6-(10-Hydroxydecyl)-2,3-dimethoxy-5-methyl-1,4-benzoquinone (idebenone) has been found to have a membrane-stabilizing activity in studies using lysosomes. Using dimyristoyl L-alpha-phosphatidylcholine (DMPC) as a substrate, the effects of idebenone and related benzoquinones on phospholipid digestion by phospholipase A(2) (PLA(2)) was investigated. Free myristic acid, released from DMPC upon PLA(2) treatment, was anthrylmethylated with 9-anthryldiazomethane (ADAM) and determined by reversed-phase HPLC. Idebenone and a related benzoquinone, 2,3-dimethoxy-5-methyl-6-(10-morpholinodecyl)-1,4-benzoquinone hydrochloride (QS-10 . Mor), inhibited the hydrolysis of the substrate with PLA(2) in a dose-dependent manner. It is suggested that the effect of idebenone on PLA(2), in addition to its antioxidant activity against lipid peroxidation, can be attributed to membrane-stabilizing activity.
引用
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页码：779 / 781
页数：3
相关论文
共 21 条
  • [1] ALLEN RC, 1987, ANNU REP MED CHEM, V22, P315
  • [2] 9-DIAZOMETHYLANTHRACENE AS A NEW FLUORESCENCE AND ULTRAVIOLET LABEL FOR THE SPECTROMETRIC DETECTION OF PICOMOLE QUANTITIES OF FATTY-ACIDS BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY
    BARKER, SA
    MONTI, JA
    CHRISTIAN, ST
    BENINGTON, F
    MORIN, RD
    [J]. ANALYTICAL BIOCHEMISTRY, 1980, 107 (01) : 116 - 123
  • [3] ENZYME TOPOLOGY OF INTRACELLULAR MEMBRANES
    DEPIERRE, JW
    ERNSTER, L
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1977, 46 : 201 - 262
  • [4] STRUCTURE OF BOVINE PANCREATIC PHOSPHOLIPASE-A2 AT 1.7A RESOLUTION
    DIJKSTRA, BW
    KALK, KH
    HOL, WGJ
    DRENTH, J
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1981, 147 (01) : 97 - 123
  • [5] ESKOLA JU, 1983, CLIN CHEM, V29, P1777
  • [6] GATT S, 1969, METHOD ENZYMOL, V14, P167
  • [7] DAMAGE TO MITOCHONDRIAL RESPIRATION CHAIN IS RELATED TO PHOSPHOLIPASE-A2 ACTIVATION CAUSED BY TUMOR-NECROSIS-FACTOR
    HIGUCHI, M
    SHIROTANI, K
    HIGASHI, N
    TOYOSHIMA, S
    OSAWA, T
    [J]. JOURNAL OF IMMUNOTHERAPY, 1992, 12 (01) : 41 - 49
  • [8] EFFECTS OF IDEBENONE AND RELATED-COMPOUNDS ON RESPIRATORY ACTIVITIES OF BRAIN MITOCHONDRIA, AND ON LIPID-PEROXIDATION OF THEIR MEMBRANES
    IMADA, I
    FUJITA, T
    SUGIYAMA, Y
    OKAMOTO, K
    KOBAYASHI, Y
    [J]. ARCHIVES OF GERONTOLOGY AND GERIATRICS, 1989, 8 (03) : 323 - 341
  • [9] SUPPRESSIVE EFFECT OF COENZYME-Q10 ON PHOSPHOLIPASE-A2 ACTIVATION IN CARDIAC-CELLS AFTER PROLONGED SWIMMING
    KOYAMA, T
    KEATISUWAN, W
    KINJO, M
    SAITO, H
    [J]. LIFE SCIENCES, 1992, 51 (14) : 1113 - 1118
  • [10] INHIBITION OF NEUTROPHIL PHOSPHOLIPASE-A2 BY PARA-BROMOPHENYLACYL BROMIDE, NORDIHYDROGUAIARETIC ACID, 5,8,11,14-EICOSATETRAENOIC ACID AND QUERCETIN
    LANNI, C
    BECKER, EL
    [J]. INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1985, 76 (03): : 214 - 217
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