COVALENT IMMOBILIZATION OF GLUCOSE-OXIDASE ON METHACRYLATE COPOLYMERS FOR USE IN AN AMPEROMETRIC GLUCOSE SENSOR

被引：32

作者：

HALL, CE ^{[1
]}

HALL, EAH ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,INST BIOTECHNOL,TENNIS COURT RD,CAMBRIDGE CB2 1QT,ENGLAND

来源：

ANALYTICA CHIMICA ACTA | 1993年 / 281卷 / 03期

关键词：

AMPEROMETRY; SENSORS; COVALENT IMMOBILIZATION; GLUCOSE SENSORS; GLUCOSE OXIDASE; METHACRYLATE COPOLYMERS;

D O I：

10.1016/0003-2670(93)85026-G

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Glucose oxidase was covalently immobilised on the surface of copolymers of methyl and glycidyl methacrylates. The enzyme modified polymers were deposited from dichloromethane solutions onto platinum electrodes to form an amperometric glucose sensor based on the electrochemical measurement of hydrogen peroxide. The enzyme has been shown to retain its activity throughout the immobilisation and casting procedure, and the influence of various experimental variables have been explored for optimum analytical performance.

引用

页码：645 / 653

页数：9

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