A COSMID CONTIG AND HIGH-RESOLUTION RESTRICTION MAP OF THE 2 MEGABASE REGION CONTAINING THE HUNTINGTONS-DISEASE GENE

被引：75

作者：

BAXENDALE, S

MACDONALD, ME

MOTT, R

FRANCIS, F

LIN, C

KIRBY, SF

JAMES, M

ZEHETNER, G

HUMMERICH, H

VALDES, J

COLLINS, FS

DEAVEN, LJ

GUSELLA, JF

LEHRACH, H

BATES, GP

机构：

[1] HARVARD UNIV,MASSACHUSETTS GEN HOSP,NEUROGENET LAB,BOSTON,MA 02114

[2] HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02114

[3] UNIV MICHIGAN,DEPT INTERNAL MED & HUMAN GENET,ANN ARBOR,MI 48109

[4] UNIV MICHIGAN,HOWARD HUGHES MED INST,ANN ARBOR,MI 48109

[5] LOS ALAMOS NATL LAB,LOS ALAMOS,NM 87545

来源：

NATURE GENETICS | 1993年 / 4卷 / 02期

关键词：

D O I：

10.1038/ng0693-181

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The quest for the mutation responsible for Huntington's disease (HD) has required an exceptionally detailed analysis of a large part of 4p16.3 by molecular genetic techniques, making this stretch of 2.2 megabases one of the best characterized regions of the human genome. Here we describe the construction of a cosmid and Pl clone contig spanning the region containing the HD gene, and the establishment of a detailed, high resolution restriction map. This ordered clone library has allowed the identification of several genes from the region, and has played a vital role in the recent identification of the Huntington's disease gene. The restriction map provides the framework for the detailed analysis of a region extremely rich in coding sequences. This study also exemplifies many of the strategies to be used in the analysis of larger regions of the human genome.

引用

页码：181 / &

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