A COSMID CONTIG AND HIGH-RESOLUTION RESTRICTION MAP OF THE 2 MEGABASE REGION CONTAINING THE HUNTINGTONS-DISEASE GENE

被引：75
作者：
BAXENDALE, S
MACDONALD, ME
MOTT, R
FRANCIS, F
LIN, C
KIRBY, SF
JAMES, M
ZEHETNER, G
HUMMERICH, H
VALDES, J
COLLINS, FS
DEAVEN, LJ
GUSELLA, JF
LEHRACH, H
BATES, GP
机构：
[1] HARVARD UNIV,MASSACHUSETTS GEN HOSP,NEUROGENET LAB,BOSTON,MA 02114
[2] HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02114
[3] UNIV MICHIGAN,DEPT INTERNAL MED & HUMAN GENET,ANN ARBOR,MI 48109
[4] UNIV MICHIGAN,HOWARD HUGHES MED INST,ANN ARBOR,MI 48109
[5] LOS ALAMOS NATL LAB,LOS ALAMOS,NM 87545
来源：
NATURE GENETICS | 1993年 / 4卷 / 02期
关键词：
D O I：
10.1038/ng0693-181
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The quest for the mutation responsible for Huntington's disease (HD) has required an exceptionally detailed analysis of a large part of 4p16.3 by molecular genetic techniques, making this stretch of 2.2 megabases one of the best characterized regions of the human genome. Here we describe the construction of a cosmid and Pl clone contig spanning the region containing the HD gene, and the establishment of a detailed, high resolution restriction map. This ordered clone library has allowed the identification of several genes from the region, and has played a vital role in the recent identification of the Huntington's disease gene. The restriction map provides the framework for the detailed analysis of a region extremely rich in coding sequences. This study also exemplifies many of the strategies to be used in the analysis of larger regions of the human genome.
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页码：181 / &
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