MONOCLONAL-ANTIBODIES AGAINST HEPATITIS-B E-ANTIGEN - PRODUCTION, CHARACTERIZATION, AND USE FOR DIAGNOSIS

被引：5

作者：

KOREC, E

DOSTALOVA, V

KORCOVA, J

MANCAL, P

KONIG, J

BORISOVA, G

CIBINOGEN, V

PUMPEN, P

GREN, E

HLOZANEK, I

机构：

[1] INST SERA & VACCINES, PRAGUE 10, CZECHOSLOVAKIA

[2] INST HYG & EPIDEMIOL, CS-10042 PRAGUE 10, CZECHOSLOVAKIA

[3] ACAD SCI LASSR, INST ORGAN SYNTH, RIGA 226006, LATVIA, USSR

来源：

JOURNAL OF VIROLOGICAL METHODS | 1990年 / 28卷 / 02期

关键词：

ELISA; Hybridoma; Recombinant HBeAg;

D O I：

10.1016/0166-0934(90)90031-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Five different hybridoma clones secreting anti-HBeAg antibody were constructed by fusing cells of mouse myeloma line SP2/0 with splenocytes from BALB/c mice immunized with recombinant HBeAg. The monoclonal antibodies obtained were characterized immunologically and one was used to develop ELISA for detection of HBeAg and anti-HBeAg antibody. These monoclonal assays enabled the detection of 3 U HBeAg/ml and 1 U anti-HBeAg/ml with reference to standards of the Paul Ehrlich Institute, Frankfurt, F.R.G. Both assays compared well with a commercially available kit (Abbott Laboratory) and were used for detection of HBeAg and anti-HBeAg antibody in clinical serum samples. © 1990.

引用

页码：165 / 170

页数：6

共 5 条

[1]

BORISOVA GP, 1984, DOKL AKAD NAUK SSSR+, V279, P1245

[2] MONOCLONAL-ANTIBODIES TO HEPATITIS-BE ANTIGEN (HBEAG) DERIVED FROM HEPATITIS-B CORE ANTIGEN (HBCAG) - THEIR USE IN CHARACTERIZATION AND DETECTION OF HBEAG [J].